Document Detail


The fine structural localization of testicular phosphatases in man: the control testis.
MedLine Citation:
PMID:  175958     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Electron microscopic cytochemistry was used to determine the localization of five phosphatase enzymes-glucose-6-phosphatase, inosine diphosphatase, thiamine pyrophosphatase, acid phosphatase, and adenosine triphosphatase-in control human testes. Glucose-6-phosphatase occurred in the endoplasmic reticulum and nuclear envelope of Sertoli cells, Leydig cells and primitive spermatogonia, but was not observed in more advanced spermatogenic cells. The presence of glucose-6-phosphatase activity paralleled the presence of glycogen in spermatogenic cells, i.e., both occurred in type AL and AD spermatogonia but not in type AP or B spermatogonia or in more advanced spermatogenic cells. Inosine diphosphatase activity was found in the endoplasmic reticulum, nuclear envelope, and Golgi complex of Sertoli cells and all spermatogenic cells except late spermatids. Additionally, inosine diphosphatase activity was localized at the junctions between Sertoli cells and late spermatids, but was not associated with any other plasma membrane. Thiamine pyrophosphatase reaction product was found in the Golgi bodies of Sertoli cells and in spermatogenic cells through immature spermatids. Neither inosine diphosphatase nor thiamine pyrophosphatase was observed in the Golgi bodies of spermatids during acrosomal formation. Acid phosphatase activity was found in lysosomes of spermatogonia, spermatocytes, and spermatids, in lysosomes of Leydig cells, and in lysosomes, lipofuscin bodies, and Golgi cisternae of Sertoli cells. It is thought that Sertoli lysosomes play a role in the phagocytosis of degenerating germ cells; however, the role of spermatogenic or Leydig lysosomes is unknown. Adenosine triphosphatase activity occurred at the interfaces between two spermatogonia, and between Sertoli cells and spermatogonia, but was not observed in the spaces between two Sertoli cells, two spermatocytes, two spermatids, or between Sertoli cells and spermatocytes, or between Sertoli cells and spermatids.
Authors:
S S Barham; J D Berlin; R B Brackeen
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Cell and tissue research     Volume:  166     ISSN:  0302-766X     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  1976 Feb 
Date Detail:
Created Date:  1976-05-20     Completed Date:  1976-05-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  497-510     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acid Phosphatase / analysis
Adenosine Triphosphatases / analysis
Adult
Cell Membrane
Endoplasmic Reticulum / enzymology
Glucose-6-Phosphatase / analysis
Golgi Apparatus / enzymology
Histocytochemistry
Humans
Inosine
Leydig Cells / enzymology
Lysosomes / enzymology
Male
Phosphoric Monoester Hydrolases / analysis*
Sertoli Cells / enzymology
Spermatogenesis*
Spermatozoa / enzymology
Testis / enzymology*,  ultrastructure
Thiamine Pyrophosphatase / analysis
Chemical
Reg. No./Substance:
58-63-9/Inosine; EC 3.1.3.-/Phosphoric Monoester Hydrolases; EC 3.1.3.2/Acid Phosphatase; EC 3.1.3.9/Glucose-6-Phosphatase; EC 3.6.1.-/Adenosine Triphosphatases; EC 3.6.1.-/Thiamine Pyrophosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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