| The final step in 5.8S rRNA processing is cytoplasmic in Saccharomyces cerevisiae. | |
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MedLine Citation:
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PMID: 20008552 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The 18S rRNA component of yeast (Saccharomyces cerevisiae) 40S ribosomes undergoes cytoplasmic 3' cleavage following nuclear export, whereas exported pre-60S subunits were believed to contain only mature 5.8S and 25S rRNAs. However, in situ hybridization detected 3'-extended forms of 5.8S rRNA in the cytoplasm, which were lost when Crm1-dependent preribosome export was blocked by treatment with leptomycin B (LMB). LMB treatment rapidly blocked processing of 6S pre-rRNA to 5.8S rRNA, leading to TRAMP-dependent pre-rRNA degradation. The 6S pre-rRNA was coprecipitated with the 60S export adapter Nmd3 and cytoplasmic 60S synthesis factor Lsg1. The longer 5.8S+30 pre-rRNA (a form of 5.8S rRNA 3' extended by approximately 30 nucleotides) is processed to 6S by the nuclear exonuclease Rrp6, and nuclear pre-rRNA accumulated in the absence of Rrp6. In contrast, 6S to 5.8S processing requires the cytoplasmic exonuclease Ngl2, and cytoplasmic pre-rRNA accumulated in strains lacking Ngl2. We conclude that nuclear pre-60S particles containing the 6S pre-rRNA bind Nmd3 and Crm1 and are exported to the cytoplasm prior to final maturation by Ngl2. |
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Authors:
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Emma Thomson; David Tollervey |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-14 |
Journal Detail:
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Title: Molecular and cellular biology Volume: 30 ISSN: 1098-5549 ISO Abbreviation: Mol. Cell. Biol. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-01-27 Completed Date: 2010-02-12 Revised Date: 2010-09-27 |
Medline Journal Info:
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Nlm Unique ID: 8109087 Medline TA: Mol Cell Biol Country: United States |
Other Details:
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Languages: eng Pagination: 976-84 Citation Subset: IM |
Affiliation:
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Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cytoplasm
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metabolism* Fatty Acids, Unsaturated / pharmacology Karyopherins / genetics, metabolism RNA 3' End Processing* RNA Precursors / genetics*, metabolism RNA Transport RNA, Fungal / genetics*, metabolism RNA, Ribosomal, 5.8S / genetics*, metabolism RNA-Binding Proteins / genetics, metabolism Receptors, Cytoplasmic and Nuclear / genetics, metabolism Saccharomyces cerevisiae / drug effects, genetics*, metabolism Saccharomyces cerevisiae Proteins / genetics, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids, Unsaturated; 0/Karyopherins; 0/NMD3 protein, S cerevisiae; 0/RNA Precursors; 0/RNA, Fungal; 0/RNA, Ribosomal, 5.8S; 0/RNA-Binding Proteins; 0/Receptors, Cytoplasmic and Nuclear; 0/Saccharomyces cerevisiae Proteins; 0/exportin 1 protein; 87081-35-4/leptomycin B |
| Comments/Corrections | |
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