Document Detail

The fibroblast growth factor-2 antisense gene inhibits nuclear accumulation of FGF-2 and delays cell cycle progression in C6 glioma cells.
MedLine Citation:
PMID:  17306451     Owner:  NLM     Status:  MEDLINE    
Fibroblast growth factor-2 (FGF-2) is a potent heparin-binding protein with growth-promoting and anti-apoptotic activity. Transcription of the GFG/NUDT6 gene on the opposite DNA strand generates an overlapping antisense RNA (FGF-AS) implicated in the post-transcriptional regulation of FGF-2. C6 glioma cells coordinately express FGF-2 and FGF-AS mRNA in a cell cycle-dependent manner. Cellular FGF-2 immunoreactivity was also cell cycle-dependent, with marked nuclear accumulation during S-phase. Stable transfection and overexpression of the FGF-AS RNA resulted in suppression of total cellular FGF-2, and a reduction in nuclear accumulation of FGF-2 isoforms. Serum stimulation of growth-arrested wild-type cells evoked a rapid nuclear translocation of FGF-2, and cell cycle re-entry. FGF-AS transfectants, in contrast, showed a significant delay in recovery of both nuclear FGF-2 staining and S-phase re-entry. Similar results were observed when cells were released from aphidicolin-induced G1 arrest or subjected to heat shock. These findings indicate that FGF-AS RNA inhibits expression and cell cycle-dependent nuclear accumulation of FGF-2, and this is associated with a marked delay in S-phase progression. The results suggest that the endogenous FGF antisense RNA may play a significant functional role in the regulation of FGF-2 dependent cell proliferation in FGF-2 expressing cells.
Mark Baguma-Nibasheka; Audrey W Li; Paul R Murphy
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-01-21
Journal Detail:
Title:  Molecular and cellular endocrinology     Volume:  267     ISSN:  0303-7207     ISO Abbreviation:  Mol. Cell. Endocrinol.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-05     Completed Date:  2007-06-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7500844     Medline TA:  Mol Cell Endocrinol     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  127-36     Citation Subset:  IM    
Department of Physiology and Biophysics, Dalhousie University, 5850 College Street, Halifax, Nova Scotia, Canada B3H 1X5.
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MeSH Terms
Cell Cycle*
Cell Nucleus / metabolism*
Fibroblast Growth Factor 2 / genetics,  metabolism*
Fibroblast Growth Factors / genetics*,  metabolism
Gene Expression
Gene Expression Regulation
Glioma / pathology*
Protein Transport
RNA, Antisense / genetics*
RNA, Messenger / genetics,  metabolism
Subcellular Fractions
Reg. No./Substance:
0/NUDT6 protein, rat; 0/RNA, Antisense; 0/RNA, Messenger; 103107-01-3/Fibroblast Growth Factor 2; 62031-54-3/Fibroblast Growth Factors

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