| The fibrinolytic pathway of human plasma. Isolation and characterization of the plasminogen proactivator. | |
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MedLine Citation:
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PMID: 4264575 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The conversion of the plasminogen proactivator to plasminogen activator by activated Hageman factor or its fragments has been recognized as an essential step in the conversion of plasminogen to plasmin. The plasminogen proactivator has been completely separated from prekallikrein and pre-PTA, two other proenzyme substrates of activated Hageman factor or its fragments. Plasminogen proactivator, free of any contaminating proteins as assessed by disc gel electrophoresis or isoelectric focusing, revealed a single band with an isoelectric point of 8.9 corresponding in position to the Hageman factor activatable material eluted from replicate unstained gels. After conversion of plasminogen proactivator by Hageman factor fragments to the plasminogen activator, the active site of the plasminogen activator is not inhibited by C1INH and is thus readily distinguished from that of kallikrein or PTA. The plasminogen activator is susceptible to inactivation by DFP while the plasminogen proactivator is not, as has been the case for esterases having a serine in the active site. Its interaction with plasminogen is inhibited by epsilon-aminocaproic acid. |
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Authors:
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A P Kaplan; K F Austen |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of experimental medicine Volume: 136 ISSN: 0022-1007 ISO Abbreviation: J. Exp. Med. Publication Date: 1972 Dec |
Date Detail:
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Created Date: 1973-02-17 Completed Date: 1973-02-17 Revised Date: 2010-06-22 |
Medline Journal Info:
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Nlm Unique ID: 2985109R Medline TA: J Exp Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1378-93 Citation Subset: AIM; IM |
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| MeSH Terms | |
Descriptor/Qualifier:
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Aminocaproic Acids
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pharmacology Blood Coagulation Factors* / isolation & purification Chromatography, Gel Complement System Proteins Electrophoresis, Disc Factor XII* / isolation & purification Fibrinolysin* / antagonists & inhibitors Fibrinolysis* Histidine / antagonists & inhibitors Humans Isoelectric Focusing Kallikreins Kinins Molecular Weight Plasminogen / antagonists & inhibitors Serine / antagonists & inhibitors Streptokinase Thromboplastin |
| Chemical | |
Reg. No./Substance:
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0/Aminocaproic Acids; 0/Blood Coagulation Factors; 0/Kinins; 56-45-1/Serine; 71-00-1/Histidine; 9001-30-3/Factor XII; 9001-91-6/Plasminogen; 9007-36-7/Complement System Proteins; 9035-58-9/Thromboplastin; EC 3.4.-/Streptokinase; EC 3.4.21.-/Kallikreins; EC 3.4.21.7/Fibrinolysin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Exp Med ISSN: 0022-1007 ISSN: 1540-9538 Publisher: The Rockefeller University Press |
Article Information Download PDF  Copyright © 1972 by The Rockefeller University Press. Received Day: 4 Month: 8 Year: 1972 Print publication date: Day: 30 Month: 11 Year: 1972 Volume: 136 Issue: 6 First Page: 1378 Last Page: 1393 ID: 2139321 PubMed Id: 4264575 |
| THE FIBRINOLYTIC PATHWAY OF HUMAN PLASMA : ISOLATION AND CHARACTERIZATION OF THE PLASMINOGEN PROACTIVATOR | |
| Allen P. Kaplan | |
| K. Frank Austen | |
| From the Departments of Medicine, Harvard Medical School and Robert B. Brigham Hospital, Boston, Massachusetts 02120 |
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