Document Detail


A fibrinolytic metalloprotease from the fruiting bodies of an edible mushroom, Armillariella mellea.
MedLine Citation:
PMID:  10664846     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A fibrinolytic metalloprotease has been purified from the fruiting bodies of the edible honey mushroom (Armillariella mellea). The enzyme has a molecular weight of 18538.1508, as measured by MALDI-TOF mass spectrometry and includes Zn2+ ion as found by ICP/MS. The N-terminal amino acid sequence, XXYNGXTXSRQTTLV, do not match any known protein or open reading frame. It hydrolyzes fibrinogen as well as fibrin, but does not show any proteolytic activity for other blood proteins such as thrombin, human albumin, bovine albumin, human IgG, hemoglobin, or urokinase. This protease hydrolyzes both A alpha and B beta subunits of human fibrinogen with equal efficiency. The enzyme activity was strongly inhibited by EDTA and 1,10-phenanthroline, indicating that the enzyme is a metalloprotease. No inhibition was found with PMSF, E-64, pepstatin, and 2-mercaptoethanol. The activity of the purified enzyme was slightly increased by Mg2+, Zn2+, and Co2+, but the enzyme was totally inhibited by Hg2+. It has broad substrate specificity for synthetic peptides, and a pH optimum at 7, suggested that the purified enzyme was a neutral protease. It was thermally stable up to 60 degrees C and the maximum fibrinolytic activity was at 55 degrees C.
Authors:
J H Kim; Y S Kim
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  63     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  1999 Dec 
Date Detail:
Created Date:  2000-03-21     Completed Date:  2000-03-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  2130-6     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Sangji University, Wonju, Korea.
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MeSH Terms
Descriptor/Qualifier:
Agaricales / enzymology*
Amino Acid Sequence
Animals
Cattle
Chelating Agents / pharmacology
Edetic Acid / pharmacology
Electrophoresis, Polyacrylamide Gel
Fibrinolysis*
Humans
Hydrogen-Ion Concentration
Metalloendopeptidases / chemistry,  isolation & purification*,  metabolism
Molecular Sequence Data
Molecular Weight
Phenanthrolines / pharmacology
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Substrate Specificity
Temperature
Zinc / metabolism
Chemical
Reg. No./Substance:
0/Chelating Agents; 0/Phenanthrolines; 60-00-4/Edetic Acid; 66-71-7/1,10-phenanthroline; 7440-66-6/Zinc; EC 3.4.24.-/Metalloendopeptidases

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