| The ferulic acid esterases of Chrysosporium lucknowense C1: Purification, characterization and their potential application in biorefinery. | |
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MedLine Citation:
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PMID: 22133444 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Three ferulic acid esterases from the filamentous fungus Chrysosporium lucknowense C1 were purified and characterized. The enzymes were most active at neutral pH and temperatures up to 45°C. All enzymes released ferulic acid and p-coumaric acid from a soluble corn fibre fraction. Ferulic acid esterases FaeA1 and FaeA2 could also release complex dehydrodiferulic acids and dehydrotriferulic acids from corn fibre oligomers, but released only 20% of all ferulic acid present in sugar beet pectin oligomers. Ferulic acid esterase FaeB2 released almost no complex ferulic acid oligomers from corn fibre oligomers, but 60% of all ferulic acid from sugar beet pectin oligomers. The ferulic acid esterases were classified based on both, sequence similarity and their activities toward synthetic substrates. The type A ferulic acid esterases FaeA1 and FaeA2 are the first members of the phylogenetic subfamily 5 to be biochemically characterized. Type B ferulic acid esterase FaeB2 is a member of subfamily 6. |
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Authors:
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S Kühnel; L Pouvreau; M M Appeldoorn; S W A Hinz; H A Schols; H Gruppen |
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Publication Detail:
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Type: Journal Article Date: 2011-09-29 |
Journal Detail:
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Title: Enzyme and microbial technology Volume: 50 ISSN: 0141-0229 ISO Abbreviation: Enzyme Microb. Technol. Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2011-12-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8003761 Medline TA: Enzyme Microb Technol Country: United States |
Other Details:
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Languages: eng Pagination: 77-85 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
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Laboratory of Food Chemistry, Wageningen University, Bomenweg 2, 6703 HD Wageningen, The Netherlands. |
Export Citation:
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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