Document Detail


The ferulic acid esterases of Chrysosporium lucknowense C1: Purification, characterization and their potential application in biorefinery.
MedLine Citation:
PMID:  22133444     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Three ferulic acid esterases from the filamentous fungus Chrysosporium lucknowense C1 were purified and characterized. The enzymes were most active at neutral pH and temperatures up to 45°C. All enzymes released ferulic acid and p-coumaric acid from a soluble corn fibre fraction. Ferulic acid esterases FaeA1 and FaeA2 could also release complex dehydrodiferulic acids and dehydrotriferulic acids from corn fibre oligomers, but released only 20% of all ferulic acid present in sugar beet pectin oligomers. Ferulic acid esterase FaeB2 released almost no complex ferulic acid oligomers from corn fibre oligomers, but 60% of all ferulic acid from sugar beet pectin oligomers. The ferulic acid esterases were classified based on both, sequence similarity and their activities toward synthetic substrates. The type A ferulic acid esterases FaeA1 and FaeA2 are the first members of the phylogenetic subfamily 5 to be biochemically characterized. Type B ferulic acid esterase FaeB2 is a member of subfamily 6.
Authors:
S Kühnel; L Pouvreau; M M Appeldoorn; S W A Hinz; H A Schols; H Gruppen
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Publication Detail:
Type:  Journal Article     Date:  2011-09-29
Journal Detail:
Title:  Enzyme and microbial technology     Volume:  50     ISSN:  0141-0229     ISO Abbreviation:  Enzyme Microb. Technol.     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2011-12-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8003761     Medline TA:  Enzyme Microb Technol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  77-85     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
Affiliation:
Laboratory of Food Chemistry, Wageningen University, Bomenweg 2, 6703 HD Wageningen, The Netherlands.
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