| A fermentor system for regulating oxygen at low concentrations in cultures of Saccharomyces cerevisiae. | |
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MedLine Citation:
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PMID: 9501444 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The growth of yeast cells to high densities at low, but constant, oxygen concentrations is difficult because the cells themselves respire oxygen; hence, as cell mass increases, so does oxygen consumption. To circumvent this problem, we have designed a system consisting of a computer-controlled gas flow train that adjusts oxygen concentration in the gas flow to match cellular demand. It does this by using a proportional-integral-differential algorithm in conjunction with a three-way valve to mix two gases, adjusting their proportions to maintain the desired oxygen concentration. By modeling yeast cell yields at intermediate to low oxygen concentrations, we have found that cellular respiration declines with oxygen concentration, most likely because of a decrease in the expression of genes for respiratory proteins. These lowered rates of oxygen consumption, together with the gas flow system described here, allow the growth of yeast cells to high densities at low oxygen concentrations. This system can also be used to grow cells at any desired oxygen concentration and for regulated shifts between oxygen concentrations. |
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Authors:
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P V Burke; K E Kwast; F Everts; R O Poyton |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 64 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 1998 Mar |
Date Detail:
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Created Date: 1998-03-26 Completed Date: 1998-03-26 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1040-4 Citation Subset: IM |
Affiliation:
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Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309-0347, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Computers Fermentation* Gene Expression Regulation, Fungal Oxygen / analysis* Oxygen Consumption Saccharomyces cerevisiae / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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GM 30228/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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7782-44-7/Oxygen |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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