| The fenpropimorph resistance gene FEN2 from Saccharomyces cerevisiae encodes a plasma membrane H+-pantothenate symporter. | |
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MedLine Citation:
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PMID: 10373490 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The product of the FEN2 gene of Saccharomyces cerevisiae has previously been described as a protein conferring sensitivity to the antifungal agent fenpropimorph. Fen2p was postulated to act as a common regulator of carbon and nitrogen catabolite repression and of amino acid and ergosterol biosynthesis. In this paper, we present experimental evidence characterizing Fen2p as a plasma membrane-localized transporter for the vitamin pantothenate. The high affinity transport system (Km = 3.5 microM) is sensitive to uncouplers, suggesting a H+-pantothenate cotransport. Pantothenate transport rates in yeast are modulated by extracellular pantothenate, being maximal at low pantothenate concentrations. It is demonstrated that beta-alanine can suppress the growth defect of FEN2 wild-type and fen2 mutant cells on pantothenate-free medium. Evidence is presented that beta-alanine is transported by the general amino acid permease Gap1p. The relation among pantothenate transport, nitrogen catabolite repression, and sensitivity to the antifungal agent fenpropimorph is discussed. |
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Authors:
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J Stolz; N Sauer |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 274 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1999 Jun |
Date Detail:
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Created Date: 1999-07-15 Completed Date: 1999-07-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 18747-52 Citation Subset: IM |
Affiliation:
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Lehrstuhl Botanik II, Molekulare Pflanzenphysiologie, Universität Erlangen-Nürnberg, Staudtstrasse 5, D-91058 Erlangen, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Biological Transport / genetics Carrier Proteins / genetics*, metabolism Cell Membrane / chemistry, metabolism Drug Resistance, Microbial / genetics Fungicides, Industrial / pharmacology* Membrane Proteins / genetics*, metabolism Molecular Sequence Data Morpholines / pharmacology* Pantothenic Acid / metabolism* Phenotype Saccharomyces cerevisiae / drug effects, genetics*, metabolism Saccharomyces cerevisiae Proteins* Symporters* |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/FEN2 protein, S cerevisiae; 0/Fungicides, Industrial; 0/Membrane Proteins; 0/Morpholines; 0/Saccharomyces cerevisiae Proteins; 0/Symporters; 0/pantothenate transporter; 67306-03-0/fenpropimorph; 79-83-4/Pantothenic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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