Document Detail


The fatty acid profile changes in marine invertebrate larval cells during cryopreservation.
MedLine Citation:
PMID:  19778531     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The development of cryopreservation methods for embryonic cells and larvae of sea animals offers a great potential for marine biotechnology. Larval cells of bivalves and sea urchins were frozen to -196 degrees C using traditional cryoprotectants (Me(2)SO and trehalose) and the cryoprotective mixture developed by us. In addition to Me(2)SO and trehalose, this mixture contained an exogenous lipid extract from mussel tissues and antioxidants. A positive effect of antioxidants (alpha-tocopherol acetate, ascorbic acid or echinochrome, the quinoid pigment of sea urchins) on cell viability became significant only in the presence of exogenous lipids. Antioxidants added to cryoprotective mixtures did not reveal visible cryoprotective activity when used separately. To better understand the mechanism of the protective effect of exogenous lipids on cell membranes of sea animals, a comparative analysis of the fatty acid (FA) composition of total lipids in larval cells before and after freezing was carried out using a gas-liquid chromatography. The results indicate that freezing-thawing has direct effects on the FA composition of major lipid classes in marine invertebrate cells, and these effects can vary depending on the provenance of the cells. We have found that (I) both cell viability and the FA profile of cell lipids after cryopreservation depend on the cryoprotectants used; (II) an amount of saturated, monoenic and polyenic FAs changes significantly after cryopreservation. We assume that the addition of the exogenous lipid extract in form of liposomes could promote a renewal of disturbance areas and prevent from membrane damages during freezing-thawing.
Authors:
N A Odintsova; A V Boroda; P V Velansky; E Ya Kostetsky
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-22
Journal Detail:
Title:  Cryobiology     Volume:  59     ISSN:  1090-2392     ISO Abbreviation:  Cryobiology     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-11-30     Completed Date:  2010-02-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  335-43     Citation Subset:  IM    
Affiliation:
Laboratory of Cell Biophysics, A.V. Zhirmunsky Institute of Marine Biology, Far Eastern Branch of Russian Academy of Sciences, Palchevsky St. 17, Vladivostok 690041, Russia. nelodin@mail.ru
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Survival
Cryopreservation / methods*
Cryoprotective Agents / pharmacology
Dimethyl Sulfoxide / pharmacology
Fatty Acids / metabolism*
Freezing
Larva / drug effects,  metabolism*
Lipids / pharmacology
Membrane Fluidity
Membrane Lipids / metabolism
Mytilus / chemistry
Strongylocentrotus
Tissue Extracts / pharmacology
Trehalose / pharmacology
Chemical
Reg. No./Substance:
0/Cryoprotective Agents; 0/Fatty Acids; 0/Lipids; 0/Membrane Lipids; 0/Tissue Extracts; 67-68-5/Dimethyl Sulfoxide; 99-20-7/Trehalose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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