Document Detail


The fatty acid profile of buccal cheek cell phospholipids is a noninvasive marker of long-chain polyunsaturated Fatty Acid status in piglets.
MedLine Citation:
PMID:  12163682     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The fatty acid pattern of cheek cell phospholipids has been proposed as a noninvasive marker of long-chain polyunsaturated fatty acid (PUFA) status. However, the cheek cell phospholipid fatty acid pattern has been compared only with that of plasma and erythrocytes. The objective of this study was to assess the extent to which the fatty acid profile of cheek cell phospholipids reflects that of tissue phospholipids. Piglets (n = 31; 6 d old) were fed five formula diets differing in total fat and fatty acid composition. After 14 d of consuming the assigned diets, cheek cell plasma, erythrocyte, liver, muscle, adipose tissue, retina and brain samples were collected for determination of the phospholipid fatty acid patterns. There were significant correlations between the cheek cell phospholipid content of most PUFA and the content of these fatty acids in tissue phospholipids (r = 0.509-0.951, P < 0.01). The cheek cell phospholipid content of most of the PUFA, except 20:4(n-6), reflected that of other tissue phospholipids as well as, or nearly as well as the contents of plasma and/or erythrocyte phospholipids. The correlations between the 22:6(n-3) contents of cheek cell, plasma, or erythrocyte phospholipids and those of brain and retina phospholipids were relatively poor (r = 0.596-0.737, P < 0.001). We conclude that the fatty acid pattern of cheek cell phospholipid can be used as a noninvasive marker of PUFA status, but it is not a better index than the pattern of plasma or erythrocyte phospholipids, particularly for assessing the fatty acid pattern of organs with slow fatty acid incorporation and/or turnover rates.
Authors:
Alexandre Lapillonne; James C DeMar; Vijay Nannegari; William C Heird
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of nutrition     Volume:  132     ISSN:  0022-3166     ISO Abbreviation:  J. Nutr.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-08-06     Completed Date:  2002-09-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2319-23     Citation Subset:  IM    
Affiliation:
U.S. Department of Agriculture/ARS Children's Nutrition Research Center, Baylor College of Medicine, Houston, TX 77030-2600, USA. alapillo@bcm.tmc.edu
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MeSH Terms
Descriptor/Qualifier:
Adipose Tissue / metabolism
Animal Feed
Animals
Biological Markers
Brain / metabolism
Cheek
Diet
Fatty Acids, Nonesterified / metabolism
Fatty Acids, Unsaturated / blood,  metabolism*
Liver / metabolism
Mouth Mucosa / metabolism*
Muscle, Skeletal / metabolism
Phospholipids / metabolism*
Retina / metabolism
Swine
Grant Support
ID/Acronym/Agency:
IR01-HD37133/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Fatty Acids, Nonesterified; 0/Fatty Acids, Unsaturated; 0/Phospholipids

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