Document Detail


The fatty acid composition of human gliomas differs from that found in nonmalignant brain tissue.
MedLine Citation:
PMID:  8972462     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To compare the fatty acid composition of tumor tissue from glioma patients with that of normal brain tissue, tissue samples were obtained from 13 glioma patients and from 3 nonmalignant patients. Following lipid extraction, total fatty acid composition was measured using gas-liquid chromatography. samples were further separated into phospholipids and neutral lipids. Representative samples were then separated into phospholipid classes by thin-layer chromatography and the fatty acid composition assayed. Levels of the polyunsaturated fatty acid (PUFA) docosahexaenoic acid (DHA), were significantly reduced (P = 0.029) in the glioma samples compared with normal brain samples; mean values were 4.8 +/- 2.9% and 9.2 +/- 1.0%, respectively. This reduction in glioma DHA content was also observed in terms of phospholipids (4.6 +/- 2.1% vs. 9.6 +/- 0.8%, P = 0.002). The phosphatidylserine and phosphatidylethanolamine phospholipid classes were reduced in the glioma samples. Differences were also noted in the n-6 PUFA content between glioma and normal brain samples. The glioma content of the n-6 PUFA linoleic acid was significantly greater (P < 0.05) than that observed in the control samples in terms of total lipids. Thus, the fatty acid composition of human gliomas differs from that found in nonmalignant brain tissue.
Authors:
D D Martin; M E Robbins; A A Spector; B C Wen; D H Hussey
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Lipids     Volume:  31     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  1996 Dec 
Date Detail:
Created Date:  1997-04-01     Completed Date:  1997-04-01     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1283-8     Citation Subset:  IM    
Affiliation:
Department of Radiology, University of Iowa Hospitals and Clinics, Iowa City 52242, USA.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Aged, 80 and over
Brain Chemistry*
Brain Neoplasms / chemistry*,  diet therapy,  therapy
Child
Child, Preschool
Dietary Fats, Unsaturated / therapeutic use
Docosahexaenoic Acids / analysis
Fatty Acids / analysis*
Fatty Acids, Unsaturated / analysis,  therapeutic use
Female
Glioma / chemistry*,  diet therapy,  therapy
Humans
Lipids / analysis
Male
Middle Aged
Phospholipids / analysis
Chemical
Reg. No./Substance:
0/Dietary Fats, Unsaturated; 0/Fatty Acids; 0/Fatty Acids, Unsaturated; 0/Lipids; 0/Phospholipids; 25167-62-8/Docosahexaenoic Acids

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