Document Detail

The fate of human sperm-derived mtDNA in somatic cells.
MedLine Citation:
PMID:  9382109     Owner:  NLM     Status:  MEDLINE    
Inheritance of animal mtDNA is almost exclusively maternal, most likely because sperm-derived mitochondria are actively eliminated from the ovum, either at or soon after fertilization. How such elimination occurs is currently unknown. We asked whether similar behavior could be detected in somatic cells, by following the fate of mitochondria and mtDNAs after entry of human sperm into transformed cells containing mitochondria but lacking endogenous mtDNAs (rho0 cells). We found that a high proportion (10%-20%) of cells contained functioning sperm mitochondria soon after sperm entry. However, under selective conditions permitting only the survival of cells harboring functional mtDNAs, only approximately 1/10(5) cells containing sperm mitochondria survived and proliferated. These data imply that mitochondria in sperm can enter somatic cells relatively easily, but they also suggest that mechanisms exist to eliminate sperm-derived mtDNA from somatic cells, mechanisms perhaps similar to those presumed to operate in the fertilized oocyte.
G Manfredi; D Thyagarajan; L C Papadopoulou; F Pallotti; E A Schon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of human genetics     Volume:  61     ISSN:  0002-9297     ISO Abbreviation:  Am. J. Hum. Genet.     Publication Date:  1997 Oct 
Date Detail:
Created Date:  1997-11-07     Completed Date:  1997-11-07     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370475     Medline TA:  Am J Hum Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  953-60     Citation Subset:  IM    
H. Houston Merritt Clinical Research Center for Muscular Dystrophy and Related Disorders, and Department of Neurology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA.
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MeSH Terms
Cell Survival
Clone Cells
DNA, Mitochondrial / analysis*,  genetics
Electron Transport Complex IV / analysis,  biosynthesis
Genetic Markers
Mitochondria / metabolism*,  ultrastructure
Polymerase Chain Reaction / methods
Polymorphism, Restriction Fragment Length
Sperm-Ovum Interactions*
Spermatozoa / physiology*
Grant Support
Reg. No./Substance:
0/DNA, Mitochondrial; 0/Genetic Markers; EC Transport Complex IV

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