Document Detail


The fate of N-dansyl-L-phenylalanine in the cerebrospinal fluid after intraventricular and intracisternal injection: a comparative fluorescence microscopic and analytical study.
MedLine Citation:
PMID:  1201604     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Absorption, accumulation and release of N-Dansyl-L-phenylalanine (DPA) through the ependyma, plexus choriodei and brain parenchyma after intraventricular and intracisternal injection was examined at different postinjection intervals by fluorescence microscopy. The following results were obtained: 1. After intraventricular injection, DPA is rapidly absorbed from the ependyma and plexus choriodei in all ventricles and subsequently disappears from the various points of the ventricles at different times. DPA is no longer evident in the ependyma after 40 min and the plexus after 90 min. Aborption and storage occur primarily in the dopaminergic centers of the brain. This stage begins 5 min p.i. attains a maximum after 40 min and is maintained up to 180 min p.i. 2. If DPA is administered intracisternally, fluorescence is initially restricted to the ependyma and choroid plexus of the fourth ventricle and to the wall of the aquaeduct. Only at 5-10 min p.i. are rostral ventricular portions labelled. Passage of the amino acid out of the ventricle only occurs to a limited extent. 40 min after intracisternal injection, DPA is no longer demonstrable in the ependyma and plexus or brain parenchyma. 3. Intrathecally administered DPA appears in the periglomerular tubules of the kidney as well 2.5 min p.i. and is stored there for up to 40 min. The kidney medulla remains free of fluorescence. 4. DPA injected into the CSF is protein-bound.
Authors:
E J Rapräger; B Röder
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Cell and tissue research     Volume:  164     ISSN:  0302-766X     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  1975 Dec 
Date Detail:
Created Date:  1976-03-01     Completed Date:  1976-03-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  251-60     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood-Brain Barrier
Brain / metabolism*
Cerebral Ventricles / metabolism
Cerebrospinal Fluid Proteins / metabolism
Choroid Plexus / metabolism
Dansyl Compounds
Ependyma / metabolism
Female
Injections, Intraventricular
Kidney Tubules / metabolism
Male
Microscopy, Fluorescence
Phenylalanine / analogs & derivatives,  cerebrospinal fluid,  metabolism*
Rats
Chemical
Reg. No./Substance:
0/Cerebrospinal Fluid Proteins; 0/Dansyl Compounds; 63-91-2/Phenylalanine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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