| The fate of N-dansyl-L-phenylalanine in the cerebrospinal fluid after intraventricular and intracisternal injection: a comparative fluorescence microscopic and analytical study. | |
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MedLine Citation:
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PMID: 1201604 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Absorption, accumulation and release of N-Dansyl-L-phenylalanine (DPA) through the ependyma, plexus choriodei and brain parenchyma after intraventricular and intracisternal injection was examined at different postinjection intervals by fluorescence microscopy. The following results were obtained: 1. After intraventricular injection, DPA is rapidly absorbed from the ependyma and plexus choriodei in all ventricles and subsequently disappears from the various points of the ventricles at different times. DPA is no longer evident in the ependyma after 40 min and the plexus after 90 min. Aborption and storage occur primarily in the dopaminergic centers of the brain. This stage begins 5 min p.i. attains a maximum after 40 min and is maintained up to 180 min p.i. 2. If DPA is administered intracisternally, fluorescence is initially restricted to the ependyma and choroid plexus of the fourth ventricle and to the wall of the aquaeduct. Only at 5-10 min p.i. are rostral ventricular portions labelled. Passage of the amino acid out of the ventricle only occurs to a limited extent. 40 min after intracisternal injection, DPA is no longer demonstrable in the ependyma and plexus or brain parenchyma. 3. Intrathecally administered DPA appears in the periglomerular tubules of the kidney as well 2.5 min p.i. and is stored there for up to 40 min. The kidney medulla remains free of fluorescence. 4. DPA injected into the CSF is protein-bound. |
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Authors:
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E J Rapräger; B Röder |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Cell and tissue research Volume: 164 ISSN: 0302-766X ISO Abbreviation: Cell Tissue Res. Publication Date: 1975 Dec |
Date Detail:
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Created Date: 1976-03-01 Completed Date: 1976-03-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0417625 Medline TA: Cell Tissue Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 251-60 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood-Brain Barrier Brain / metabolism* Cerebral Ventricles / metabolism Cerebrospinal Fluid Proteins / metabolism Choroid Plexus / metabolism Dansyl Compounds Ependyma / metabolism Female Injections, Intraventricular Kidney Tubules / metabolism Male Microscopy, Fluorescence Phenylalanine / analogs & derivatives, cerebrospinal fluid, metabolism* Rats |
| Chemical | |
Reg. No./Substance:
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0/Cerebrospinal Fluid Proteins; 0/Dansyl Compounds; 63-91-2/Phenylalanine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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