Document Detail


A fast and sensitive method for detecting specific viral RNA in mammalian cells.
MedLine Citation:
PMID:  2450208     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A quick and sensitive method to quantitate viral RNA synthesis has been developed. Utilizing glutaraldehyde to fix infected cells onto nitrocellulose paper, viral RNA can be probed directly in situ. Viral message can be detected from as few as 10(4) infected cells. This technique can be used to study viral gene expression and can be adapted to screen the activity of antiviral agents such as interferon.
Authors:
U Paeratakul; P R De Stasio; M W Taylor
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of virology     Volume:  62     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1988 Apr 
Date Detail:
Created Date:  1988-04-15     Completed Date:  1988-04-15     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1132-5     Citation Subset:  IM    
Affiliation:
Department of Biology, Indiana University, Bloomington 47405.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
DNA, Viral / genetics
Encephalomyocarditis virus / genetics*
Gene Expression Regulation
Glutaral
Humans
Interferons / pharmacology
Nucleic Acid Hybridization
RNA, Viral / analysis*,  biosynthesis
Simplexvirus / genetics*
Transcription, Genetic
Vero Cells
Virology / methods*
Grant Support
ID/Acronym/Agency:
AI21898/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Viral; 0/RNA, Viral; 111-30-8/Glutaral; 9008-11-1/Interferons
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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