| The failure of selenium supplementation to prevent copper-induced liver damage in Fischer 344 rats. | |
| | |
MedLine Citation:
|
PMID: 11346254 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
This study evaluates the ability of selenium (Se) supplementation to prevent experimental copper (Cu)-induced hepatocellular damage. Weanling male Fischer 344 rats were randomly assigned to groups of 15, 3 groups (A,B,C) were fed Cu-loaded diets (containing 2000 microg/g copper, added as CuSO4) and different levels of Se (added as Na2SeO3 x 5H2O) as follows: A) Cu-loaded/Se adequate diet (0.4 microg/g Se, fed basis); B) Cu-loaded/Se-supplemented diet (2 microg/g Se); and C) Cu-loaded/Se-deficient diet (< 0.2 microg/g). Three additional groups (D,E,F) were fed diets containing adequate levels of Cu (14 microg/g Cu, fed basis) and different levels of Se as follows: D) Cu-adequate/Se-adequate diet; E) Cu-adequate/Se-supplemented diet (2 microg/g Se); and F) Cu-adequate/Se-deficient (< 0.2 microg/g) diet. After 4, 8, and 12 weeks on the experimental diets, liver samples were processed for histology, histochemistry, metal analysis, glutathione peroxidase (GSH-Px) measurement, and quantification of malondialdehyde (MDA). Morphologic changes characteristic of Cu-associated hepatitis, without an increase in hepatic MDA levels, were seen in all Cu-loaded rats in each sampling. Similar changes occurred in rats fed Se-adequate, Se-supplemented and Se-deficient diets. This study demonstrates that Fischer 344 rats fed 2000 microg/g Cu develop morphologic changes due to Cu toxicity without evidence of lipid peroxidation. Furthermore, Se supplementation does not result in protection against Cu-induced liver injury. |
| | |
Authors:
|
E M Aburto; A Cribb; I C Fuentealba; B O Ikede; F S Kibenge; F Markham |
Related Documents
:
|
9032204 - Effect of free radical scavengers on diaphragmatic fatigue. 6711484 - Dietary selenium levels needed to maintain balance in north american adults consuming s... 7709594 - Fusarium proliferatum-fermented corn stimulates development of placental glutathione s-... 7048274 - Dietary effects on selenite toxicity in the chick. 16050124 - Utilization of metabolizable energy by young broilers and birds of intermediate growth ... 8648434 - D-tagatose is a bulk sweetener with zero energy determined in rats. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Canadian journal of veterinary research = Revue canadienne de recherche vétérinaire Volume: 65 ISSN: 0830-9000 ISO Abbreviation: Can. J. Vet. Res. Publication Date: 2001 Apr |
Date Detail:
|
Created Date: 2001-05-10 Completed Date: 2001-06-07 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 8607793 Medline TA: Can J Vet Res Country: Canada |
Other Details:
|
Languages: eng Pagination: 104-10 Citation Subset: IM |
Affiliation:
|
Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Copper / metabolism, toxicity* Dietary Supplements Glutathione Peroxidase / analysis Histocytochemistry / veterinary Lipid Peroxidation / drug effects Liver / drug effects*, metabolism, pathology Male Malondialdehyde / analysis Pilot Projects Random Allocation Rats Rats, Inbred F344 Selenium / administration & dosage* Time Factors |
| Chemical | |
Reg. No./Substance:
|
542-78-9/Malondialdehyde; 7440-50-8/Copper; 7782-49-2/Selenium; EC 1.11.1.9/Glutathione Peroxidase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Diagnostic superiority of continuous-loop electrocardiographic recording over other testing in a pat...
Next Document: Relationships among scrotal and testicular characteristics, sperm production, and seminal quality in...