Document Detail

A factor(s) secreted from MIN-6 beta-cells stimulates differentiation of definitive endoderm enriched embryonic stem cells towards a pancreatic lineage.
MedLine Citation:
PMID:  20674663     Owner:  NLM     Status:  MEDLINE    
In the mouse the developing pancreas is controlled by contact with, and signalling molecules secreted from, surrounding cells. These factors are best studied using explant cultures of embryonic tissue. The present study was undertaken to determine whether embryonic stem (ES) cells could be used as an alternative model in vitro system to investigate the role of cell-cell interactions in the developing pancreas. Transwell culture experiments showed that MIN-6 beta-cells secreted a factor or factors that promoted differentiation of ES cell derived definitive endoderm enriched cells towards a pancreatic fate. Further studies using MIN-6 condition medium showed that the factor(s) involved was restricted to MIN-6 cells, could be concentrated with ammonium sulphate, and was sensitive to heat treatment, suggesting that it was a protein or peptide. Further analyses showed that insulin or proinsulin failed to mimic the effects of the conditioned media. Collectively, these results suggest that beta-cells secrete a factor(s) capable of controlling their own differentiation and maturation. The culture system described here presents unique advantages in the identification and characterisation of these factors.
Daniela S Uroić; Grégory Baudouin; Laura A Ferguson; Hilary M Docherty; Ludovic Vallier; Kevin Docherty
Related Documents :
23917223 - Sox2 promotes dedifferentiation and imparts stem cell-like features to pancreatic cance...
15194443 - Importin alpha/beta-mediated nuclear protein import is regulated in a cell cycle-depend...
12915273 - Conformon-driven biopolymer shape changes in cell modeling.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-07-30
Journal Detail:
Title:  Molecular and cellular endocrinology     Volume:  328     ISSN:  1872-8057     ISO Abbreviation:  Mol. Cell. Endocrinol.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-06     Completed Date:  2011-01-18     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  7500844     Medline TA:  Mol Cell Endocrinol     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  80-6     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Elsevier Ireland Ltd. All rights reserved.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Culture Techniques
Cell Differentiation / drug effects*
Cell Line
Cell Separation
Culture Media, Conditioned / metabolism,  pharmacology
Dose-Response Relationship, Drug
Embryoid Bodies / cytology,  drug effects,  physiology
Embryonic Stem Cells / drug effects*,  physiology
Endoderm / cytology,  drug effects*,  physiology
Gene Expression Regulation, Developmental / drug effects
Insulin-Secreting Cells / physiology,  secretion*
Intercellular Signaling Peptides and Proteins / pharmacology*,  secretion
Pancreas / cytology,  drug effects,  embryology,  physiology*
Grant Support
G0701448//Medical Research Council; //Medical Research Council
Reg. No./Substance:
0/Culture Media, Conditioned; 0/Intercellular Signaling Peptides and Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Influence of soil properties on molybdenum uptake and elimination kinetics in the earthworm Eisenia ...
Next Document:  Gonadotropins and gonadotropin receptors--evolutional genetics, signalling mechanisms, extra gonadal...