| Dock/Nck facilitates PTP61F/PTP1B regulation of insulin signaling. | |
| | |
MedLine Citation:
|
PMID: 21707536 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Protein tyrosine phosphatase 1B (PTP1B) is a negative regulator of insulin receptor (IR) activation and glucose homeostasis, but the precise molecular mechanisms governing PTP1B substrate selectivity and the regulation of insulin signaling remain unclear. Here we have taken advantage of Drosophila as a model organism to establish the role of the SH3/SH2 adaptor protein Dreadlocks (Dock) and its mammalian counterpart Nck in IR regulation by PTPs. We demonstrate that the PTP1B orthologue PTP61F dephosphorylates the Drosophila IR in S2 cells in vitro and attenuates IR-induced eye overgrowth in vivo. Our studies indicate that Dock forms a stable complex with PTP61F and that Dock/PTP61F associate with the IR in response to insulin. We report that Dock is required for effective IR dephosphorylation and inactivation by PTP61F in vitro and in vivo. Furthermore, we demonstrate that Nck interacts with PTP1B and that the Nck/PTP1B complex inducibly associates with the IR for the attenuation of IR activation in mammalian cells. Our studies reveal for the first time that the adaptor protein Dock/Nck attenuates insulin signaling by recruiting PTP61F/PTP1B to its substrate the IR. |
| | |
Authors:
|
Chia-Lun Wu; Bree Buszard; Chun-Hung Teng; Wei-Lin Chen; Coral Warr; Tony Tiganis; Tzu-Ching Meng |
Related Documents
:
|
6104626 - The dual effect of alloxan modulated by 3-o-methylglucose or somatostatin on insulin se... 15582196 - Biochemical study on the hypoglycemic effects of onion and garlic in alloxan-induced di... 11785776 - Changes in pancreatic lysosomal enzymes activity as the potential factors leading to di... 3418826 - The functional effect of mild outlet obstruction on the rabbit urinary bladder. 10166556 - Diabetes- and thyroid-related eye disease. 17630266 - Diabetic nephropathy in 27,805 children, adolescents, and adults with type 1 diabetes: ... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-6-27 |
Journal Detail:
|
Title: The Biochemical journal Volume: - ISSN: 1470-8728 ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
|
Created Date: 2011-6-28 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 2984726R Medline TA: Biochem J Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Optimized native gel systems for separation of thylakoid protein complexes: novel super- and megacom...
Next Document: A new HLA-C*07 variant allele, C*07:108, identified by sequence-based typing.