| A facile system for encoding unnatural amino acids in mammalian cells. | |
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MedLine Citation:
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PMID: 19378306 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A shuttle system has been developed to genetically encode unnatural amino acids in mammalian cells using aminoacyl-tRNA synthetases (aaRSs) evolved in E. coli. A pyrrolysyl-tRNA synthetase (PylRS) mutant was evolved in E. coli that selectively aminoacylates a cognate nonsense suppressor tRNA with a photocaged lysine derivative. Transfer of this orthogonal tRNA-aaRS pair into mammalian cells made possible the selective incorporation of this unnatural amino acid into proteins. |
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Authors:
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Peng R Chen; Dan Groff; Jiantao Guo; Weijia Ou; Susan Cellitti; Bernhard H Geierstanger; Peter G Schultz |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Angewandte Chemie (International ed. in English) Volume: 48 ISSN: 1521-3773 ISO Abbreviation: Angew. Chem. Int. Ed. Engl. Publication Date: 2009 |
Date Detail:
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Created Date: 2009-05-18 Completed Date: 2009-06-23 Revised Date: 2010-09-24 |
Medline Journal Info:
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Nlm Unique ID: 0370543 Medline TA: Angew Chem Int Ed Engl Country: Germany |
Other Details:
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Languages: eng Pagination: 4052-5 Citation Subset: IM |
Affiliation:
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Department of Chemistry, The Scripps Research Institute, 10550 Torry Pines Road, La Jolla, CA 92037, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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biosynthesis*,
chemistry,
genetics* Amino Acyl-tRNA Synthetases / genetics, metabolism* Animals Archaea / genetics CHO Cells Cell Line Cricetinae Cricetulus Escherichia coli / enzymology, genetics Genetic Code* Humans Lysine / analogs & derivatives*, chemistry, genetics, metabolism Protein Biosynthesis Proteins / chemistry*, genetics |
| Grant Support | |
ID/Acronym/Agency:
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R01 GM062159/GM/NIGMS NIH HHS; R01 GM062159-08/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Proteins; 0/pyrrolysine; 56-87-1/Lysine; EC 6.1.1.-/Amino Acyl-tRNA Synthetases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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