| A facile light-emitting-diode induced fluorescence detector coupled to an integrated microfluidic device for microchip electrophoresis. | |
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MedLine Citation:
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PMID: 21530784 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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In this paper, a compact and inexpensive light emitting diode induced fluorescence (LED-IF) detector with simplified optical configuration was developed and assembled in an integrated microfluidic device for microscale electrophoresis. The facile detector mainly consisted of an LED, a focusing pinhole, an emission filter and a photodiode, and was encapsulated in the upper layer of an aluminum alloy device with two layers. At the bottom layer, integrated circuit (IC) was assembled to manipulate the voltage for sample injection and separation, LED emission and signal amplifying. A high-power LED with fan-shaped heat sink was used as excitation source. The excitation light was focused by a 1.1mm diameter pinhole fabricated in a thin piece of silver foil, and the obtained sensitivity was about 3 times as high as that using electrode plate. Other important parameters including LED driven current, fluorescence collection angle and detection distance have also been investigated. Under optimal conditions, considerable high-response of 0.09fmol and 0.18fmol mass detection limits at 0.37nL injection volume for sodium fluorescein (SF) and FITC was achieved, respectively. This device has been successfully employed to separate penicillamine (PA) enantiomers. Due to such significant features as low-cost, integration, miniaturization, and ease of commercialization, the presented microfluidic device may hold great promise for clinical diagnostics and bioanalytical applications. |
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Authors:
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Fan Yang; Xin-Chun Li; Wen Zhang; Jian-Bin Pan; Zuan-Guang Chen |
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Publication Detail:
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Type: Journal Article Date: 2011-03-16 |
Journal Detail:
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Title: Talanta Volume: 84 ISSN: 1873-3573 ISO Abbreviation: Talanta Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-05-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2984816R Medline TA: Talanta Country: England |
Other Details:
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Languages: eng Pagination: 1099-106 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier B.V. All rights reserved. |
Affiliation:
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School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006, PR China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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