Document Detail

An ezrin-rich, rigid uropod-like structure directs movement of amoeboid blebbing cells.
MedLine Citation:
PMID:  21444753     Owner:  NLM     Status:  MEDLINE    
Melanoma cells can switch between an elongated mesenchymal-type and a rounded amoeboid-type migration mode. The rounded 'amoeboid' form of cell movement is driven by actomyosin contractility resulting in membrane blebbing. Unlike elongated A375 melanoma cells, rounded A375 cells do not display any obvious morphological front-back polarisation, although polarisation is thought to be a prerequisite for cell movement. We show that blebbing A375 cells are polarised, with ezrin (a linker between the plasma membrane and actin cytoskeleton), F-actin, myosin light chain, plasma membrane, phosphatidylinositol (4,5)-bisphosphate and β1-integrin accumulating at the cell rear in a uropod-like structure. This structure does not have the typical protruding shape of classical leukocyte uropods, but, as for those structures, it is regulated by protein kinase C. We show that the ezrin-rich uropod-like structure (ERULS) is an inherent feature of polarised A375 cells and not a consequence of cell migration, and is necessary for cell invasion. Furthermore, we demonstrate that membrane blebbing is reduced at this site, leading to a model in which the rigid ezrin-containing structure determines the direction of a moving cell through localised inhibition of membrane blebbing.
Anna Lorentzen; Jeffrey Bamber; Amine Sadok; Ilan Elson-Schwab; Christopher J Marshall
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cell science     Volume:  124     ISSN:  1477-9137     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-03-29     Completed Date:  2011-09-02     Revised Date:  2014-03-19    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  1256-67     Citation Subset:  IM    
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MeSH Terms
Cell Line, Tumor
Cell Membrane / genetics,  metabolism*
Cell Movement*
Cell Polarity*
Cells / cytology*,  metabolism
Cytoskeletal Proteins / genetics,  metabolism*
Melanoma / genetics,  metabolism,  physiopathology*
Grant Support
10433//Cancer Research UK; 10437//Cancer Research UK; 3955//Cancer Research UK
Reg. No./Substance:
0/Cytoskeletal Proteins; 0/ezrin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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