Document Detail


The extracorporeal perfusion of swine uterus as an experimental model: the effect of oxytocic drugs.
MedLine Citation:
PMID:  14517766     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective of this study was to establish an experimental model for extracorporeal perfusion of swine uterus. In order to validate this model, we examined some biochemical parameters and determined the effect of oxytocic drugs (Oxytoxin, Prostaglandin E (2)) on extracorporeal perfused swine uteri. Thirty swine uteri were perfused with Krebs-Ringer bicarbonate-glucose buffer for a period up to eleven hours with the aim to preserve a viable organ, which should be responsive to hormones. The intrauterine pressure was recorded after administration of various concentrations of oxytocin and prostaglandin E (2). Perfusate pH, perfusate lactate, partial oxygen and carbon dioxide tensions, oxygen saturation, and hydrogencarbonate levels in the perfusate, all indicators of tissue ischemia or cell necrosis, showed good preservation of the organ for up to seven hours. We examined the relation of intrauterine pressure to oxytocin and prostaglandin E (2). Both were able to induce contractions of the uterus, whereas prostaglandin E (2) produced rhythmical contractions of smaller amplitude and a higher frequency. We could demonstrate that our perfusion system was able to preserve the swine uterus in a functional condition appropriate for the study of physiological questions.
Authors:
R Dittrich; T Maltaris; A Müller; C Dragonas; F Scalera; M W Beckmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et métabolisme     Volume:  35     ISSN:  0018-5043     ISO Abbreviation:  Horm. Metab. Res.     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-09-30     Completed Date:  2004-06-17     Revised Date:  2009-02-19    
Medline Journal Info:
Nlm Unique ID:  0177722     Medline TA:  Horm Metab Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  517-22     Citation Subset:  IM    
Affiliation:
University of Erlangen/Nuremberg, Department of Obstetrics and Gynecology, Erlangen, Germany. ralf.dittrich@gyn.med.uni-erlangen
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MeSH Terms
Descriptor/Qualifier:
Animals
Dinoprostone / physiology*
Female
Models, Animal
Muscle Contraction / drug effects,  physiology*
Myometrium / drug effects,  physiology*
Organ Culture Techniques / methods
Oxytocics / pharmacology
Oxytocin / physiology*
Perfusion / methods*
Swine
Tissue Preservation / methods
Uterus / drug effects,  metabolism
Chemical
Reg. No./Substance:
0/Oxytocics; 363-24-6/Dinoprostone; 50-56-6/Oxytocin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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