Document Detail


The extent of postmortem drug redistribution in a rat model.
MedLine Citation:
PMID:  10486948     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this study was to investigate the postmortem redistribution of several drugs in a rat model and to examine if any of the pharmacological properties was related to the extent of this phenomenon. One of the following drugs: phenobarbital (phenobarbitone), acetaminophen (paracetamol), carbamazepine, codeine, verapamil, amphetamine, mianserin, trimeprazine (alimemazine) or chloroquine was administered together with nortriptyline orally to rats 90 min prior to sacrifice. Heart blood was sampled immediately before sacrifice and after 2 h postmortem, as it has previously been shown that this is sufficient time for postmortem concentration changes to occur in heart blood. Blood was also sampled from the clamped abdominal inferior vena cava (representing peripheral blood) and tissue samples were taken from lungs, myocardium, liver, kidney, thigh muscle, forebrain, and vitreous humor together with a specimen from the minced carcass. Drugs were analyzed by high performance liquid or gas chromatography. For phenobarbital, acetaminophen and carbamazepine the postmortem to antemortem blood drug concentration ratios were close to 1.0 and tissue concentrations were low. The postmortem to antemortem heart blood drug concentration ratio for chloroquine (6.9 +/- 1.5) was higher than for nortriptyline (3.5 +/- 0.3), and the remaining drugs (codeine, verapamil, amphetamine, mianserin, and trimeprazine) showed ratios of the same magnitude as nortriptyline. The postmortem to antemortem blood drug concentration ratios for both heart blood and blood from the vena cava and also the lung to antemortem blood drug concentration ratio were closely related to the apparent volume of distribution for the drugs studied (p < 0.001). Accordingly, an apparent volume of distribution of more than 3-4 L/kg is a good predictor that a drug is liable to undergo postmortem redistribution with significant increments in blood levels. The postmortem drug concentration in blood from vena cava was closely related to the antemortem blood level, confirming that among the postmortem samples, the peripheral blood sample was the most representative for the antemortem blood concentration.
Authors:
T Hilberg; A Ripel; L Slørdal; A Bjørneboe; J Mørland
Related Documents :
12061628 - Characterization of spotted fever group rickettsiae detected in dogs and ticks in okina...
19389888 - A prospective study of causes of haemolysis during venepuncture: tourniquet time should...
1665018 - Electrochemical determination of azidothymidine in human whole blood.
7856878 - Glutathione disulfide variability in normal human blood.
6635908 - Development of a bovine stroma-free hemoglobin solution as a blood substitute.
8141328 - Inhibition of renal vascular 20-hete production impairs autoregulation of renal blood f...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of forensic sciences     Volume:  44     ISSN:  0022-1198     ISO Abbreviation:  J. Forensic Sci.     Publication Date:  1999 Sep 
Date Detail:
Created Date:  1999-09-30     Completed Date:  1999-09-30     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0375370     Medline TA:  J Forensic Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  956-62     Citation Subset:  IM    
Affiliation:
National Institute of Forensic Toxicology, Oslo, Norway.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Forensic Medicine / methods
Male
Pharmaceutical Preparations / metabolism*
Pharmacokinetics*
Postmortem Changes*
Rats
Rats, Wistar
Tissue Distribution
Chemical
Reg. No./Substance:
0/Pharmaceutical Preparations

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Potassium nitrite reaction with 11-nor-delta 9-tetrahydrocannabinol-9-carboxylic acid in urine in re...
Next Document:  Graphical tools for RFLP measurement quality assurance: laboratory performance charts.