Document Detail


An extended microsatellite set for linkage mapping in the domestic dog.
MedLine Citation:
PMID:  17406025     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The extremes of phenotype displayed by the domestic dog, as well as the largest number of naturally occurring inherited diseases in any mammalian species except man (>450), have generated a large interest in genomic linkage mapping in the species. Marker sets for linkage mapping should ideally show both high levels of polymorphism among the target group of animals and an even spacing of markers across the whole genome. Currently a microsatellite marker set known as Minimal Screening Set 2 (MSS2) is widely used. Here, we have extended this marker set by filling in gaps as noted from the marker positions in the CanFam genome assembly (1.0) and the 5000cR radiation hybrid (RH) map. An additional 183 markers have been positioned to increase the coverage of the MSS2 set wherever it contains a gap >9 mb or 1000(5000) RH units. We have called the marker set derived from the MSS2 set and these 183 markers, MSS3. The average physical spacing of markers in the complete 507 marker MSS3 set is 5 mb, whereas average heterozygosity of the 183 new markers on a panel of 10 dogs of differing breeds is 0.74. This marker group will allow genome-wide scans in the dog to be conducted at close to 5 cM resolution.
Authors:
David R Sargan; Jesus Aguirre-Hernandez; Francis Galibert; Elaine A Ostrander
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't     Date:  2007-04-03
Journal Detail:
Title:  The Journal of heredity     Volume:  98     ISSN:  0022-1503     ISO Abbreviation:  J. Hered.     Publication Date:    2007 May-Jun
Date Detail:
Created Date:  2007-06-20     Completed Date:  2007-09-14     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  0375373     Medline TA:  J Hered     Country:  United States    
Other Details:
Languages:  eng     Pagination:  221-31     Citation Subset:  IM    
Affiliation:
Centre for Veterinary Science, Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, UK. drs20@cam.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Chromosome Mapping*
DNA Primers
Dogs
Genetic Markers
Linkage (Genetics)*
Microsatellite Repeats / genetics*
Grant Support
ID/Acronym/Agency:
K05 CA90754/CA/NCI NIH HHS; R01 CA92167/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/DNA Primers; 0/Genetic Markers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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