Document Detail

The expression of "tissue" transglutaminase in two human cancer cell lines is related with the programmed cell death (apoptosis).
MedLine Citation:
PMID:  1679009     Owner:  NLM     Status:  MEDLINE    
The expression of "tissue" transglutaminase (tTG) in two human tumor cell lines (the cervix adenocarcinoma line HeLa-TV and the neuroblastoma cells SK-N-BE-2) was found to be in correlation with the rate of physiological cell death (apoptosis) in culture. We investigated the effect of retinoic acid (RA) and alpha-difluoromethylornithine (DFMO) in order to elucidate the relationship between tTG expression and apoptosis. RA led to a 6-fold increase of tTG activity in HeLa-TV cells and to a 12-fold increase in SK-N-BE(2) cells, which was paralleled in both cell lines by a proportional increase in the number of apoptotic bodies recovered from the cultures. On the contrary, DFMO determined a dramatic reduction of tTG expression and of the apoptotic index. Immunohistochemical analysis using an anti-tTG antibody showed that the enzyme was accumulated in both cell lines within typical apoptotic bodies. Immunocytochemistry and cell cloning of SK-N-BE(2) line demonstrated that tTG was absent in cells showing neurite outgrowth, indicating that the enzyme expression is not associated with neural differentiation, even though both phenomena are elicited by retinoic acid. On the whole, these data indicate that also in tumors tTG activation takes place in cells undergoing apoptosis. The enzyme is activated in apoptotic cells to form cross-linked protein envelopes which are insoluble in detergents and chaotropic agents. The number of insoluble protein envelopes as well as the N,N-bis(gamma-glutamyl)polyamine cross-links is related with both tTG expression and apoptotic index, strongly suggesting the participation of the enzyme in the apoptotic program.(ABSTRACT TRUNCATED AT 250 WORDS)
M Piacentini; L Fesus; M G Farrace; L Ghibelli; L Piredda; G Melino
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of cell biology     Volume:  54     ISSN:  0171-9335     ISO Abbreviation:  Eur. J. Cell Biol.     Publication Date:  1991 Apr 
Date Detail:
Created Date:  1991-10-03     Completed Date:  1991-10-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7906240     Medline TA:  Eur J Cell Biol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  246-54     Citation Subset:  IM    
Department of Biology, Tor Vergata University of Rome, Italy.
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MeSH Terms
Cell Survival / drug effects,  physiology*
Eflornithine / pharmacology
Hela Cells
Polyamines / metabolism
Transglutaminases / metabolism*
Tretinoin / pharmacology
Tumor Cells, Cultured
Reg. No./Substance:
0/Polyamines; 302-79-4/Tretinoin; 70052-12-9/Eflornithine; EC

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