Document Detail


The expression profile of the Tuber borchii nitrite reductase suggests its positive contribution to host plant nitrogen nutrition.
MedLine Citation:
PMID:  17082947     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ectomycorrhizal symbiosis is a ubiquitous association between plant roots and numerous fungal species. One of the main aspects of the ectomycorrhizal association are the regulation mechanisms of fungal genes involved in nitrogen acquisition. We report on the genomic organisation of the nitrate gene cluster and functional regulation of tbnir1, the nitrite reductase gene of the ectomycorrhizal ascomycete Tuber borchii. The sequence data demonstrate that clustering also occurs in this ectomycorrhizal fungus. Within the TBNIR1 protein sequence, we identified three functional domains at conserved positions: the FAD box, the NADPH box and the two (Fe/S)-siroheme binding site signatures. We demonstrated that tbnir1 presents an expression pattern comparable to that of nitrate transporter. In fact, we found a strong down-regulation in the presence of primary nitrogen sources and a marked tbnir1 mRNA accumulation following transfer to either nitrate or nitrogen limited conditions. The real-time PCR assays of tbnir1 and nitrate transporter revealed that both nitrate transporter and nitrite reductase expression levels are about 15-fold and 10-fold higher in ectomycorrhizal tissues than in control mycelia, respectively. The results reported herein suggest that the symbiotic fungus Tuber borchii contributes to improving the host plant's ability to make use of nitrate/nitrite in its nitrogen nutrition.
Authors:
M Guescini; S Zeppa; R Pierleoni; D Sisti; L Stocchi; V Stocchi
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Publication Detail:
Type:  Journal Article     Date:  2006-11-03
Journal Detail:
Title:  Current genetics     Volume:  51     ISSN:  0172-8083     ISO Abbreviation:  Curr. Genet.     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2006-12-07     Completed Date:  2007-03-26     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8004904     Medline TA:  Curr Genet     Country:  United States    
Other Details:
Languages:  eng     Pagination:  31-41     Citation Subset:  IM    
Affiliation:
Institute of Biological Chemistry G. Fornaini, University of Urbino Carlo Bo, Via Saffi, 2, 61029 Urbino (PU), Italy.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Anion Transport Proteins / genetics,  metabolism
Ascomycota / enzymology*,  genetics,  growth & development
Down-Regulation
Gene Expression Regulation, Fungal*
Host-Parasite Interactions
Molecular Sequence Data
Mycorrhizae / enzymology*,  genetics,  growth & development
Nitrite Reductases / genetics*,  metabolism
Nitrogen / metabolism*
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Symbiosis / genetics*,  physiology
Chemical
Reg. No./Substance:
0/Anion Transport Proteins; 0/RNA, Messenger; 0/nitrate transporters; 7727-37-9/Nitrogen; EC 1.7.-/Nitrite Reductases

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