Document Detail

The expression of placental-type glutathione S-transferase (GST-pi) in human cutaneous carcinoma in situ, that is, actinic keratosis and Bowen's disease, compared with normal human skin.
MedLine Citation:
PMID:  7586130     Owner:  NLM     Status:  MEDLINE    
The expression of human placental type glutathione S-transferase (GST-pi) was investigated in human cutaneous carcinoma in situ, that is, actinic keratosis and Bowen's disease, and compared with that in normal skin, using Northern blot and immunohistochemical analysis. In Northern blot examination, the expression of GST-pi transcript was recognized in all instances. Carcinoma in situ showed significantly higher expressions of GST-pi than normal skin. In the immunohistochemical examination, nuclear staining of GST-pi was noticed in some dysplastic cells of carcinoma in situ, especially in Bowen's disease. In actinic keratosis, a framework appearance was noticed in the staining pattern at a lower magnification because the lower part of the cytoplasm of dysplastic cells lining the stratum basale was positive for GST-pi, and all cells of the stratum granulosum and more cells of the stratum spinosum showed stronger GST-pi positive reaction than normal skin. In Bowen's disease, GST-pi positive, dysplastic cells existed throughout the epidermis. Because GST-pi positive, dysplastic cells and GST-pi positive, normal looking squamous cells made the GST-pi positive cell nests throughout the epidermis, and GST-pi positive, dysplastic cells, and GST-pi negative, normal looking cells coexisted in the parabasal layer, they showed a sawtooth appearance in the staining pattern at a lower magnification. These findings suggest that GST-pi is involved in the process of carcinogenesis.
K Shimizu; F Toriyama; H M Zhang; H Yoshida
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Carcinogenesis     Volume:  16     ISSN:  0143-3334     ISO Abbreviation:  Carcinogenesis     Publication Date:  1995 Oct 
Date Detail:
Created Date:  1995-11-28     Completed Date:  1995-11-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8008055     Medline TA:  Carcinogenesis     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2327-30     Citation Subset:  IM    
Division of Dermatology, Nagasaki Chuo National Hospital, Omura, Japan.
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MeSH Terms
Aged, 80 and over
Bowen's Disease / enzymology*,  pathology
Carcinoma in Situ / enzymology*,  pathology
Glutathione Transferase / analysis,  biosynthesis*
Isoenzymes / analysis,  biosynthesis
Keratosis / enzymology*,  pathology
Middle Aged
Placenta / enzymology
Skin / cytology,  enzymology*,  pathology
Skin Neoplasms / enzymology*,  pathology
Reg. No./Substance:
0/Isoenzymes; EC Transferase

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