| The expression of nifB gene from Herbaspirillum seropedicae is dependent upon the NifA and RpoN proteins. | |
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MedLine Citation:
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PMID: 17473889 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The putative nifB promoter region of Herbaspirillum seropedicae contained two sequences homologous to NifA-binding site and a -24/-12 type promoter. A nifB::lacZ fusion was assayed in the backgrounds of both Escherichia coli and H. seropedicae. In E. coli, the expression of nifB::lacZ occurred only in the presence of functional rpoN and Klebsiella pneumoniae nifA genes. In addition, the integration host factor (IHF) stimulated the expression of the nifB::lacZ fusion in this background. In H. seropedicae, nifB expression occurred only in the absence of ammonium and under low levels of oxygen, and it was shown to be strictly dependent on NifA. DNA band shift experiments showed that purified K. pneumoniae RpoN and E. coli IHF proteins were capable of binding to the nifB promoter region, and in vivo dimethylsulfate footprinting showed that NifA binds to both NifA-binding sites. These results strongly suggest that the expression of the nifB promoter of H. seropedicae is dependent on the NifA and RpoN proteins and that the IHF protein stimulates NifA activation of nifB promoter. |
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Authors:
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Fabiane G M Rego; Fábio O Pedrosa; Leda S Chubatsu; M Geoffrey Yates; Roseli Wassem; Maria B R Steffens; Liu U Rigo; Emanuel M Souza |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Canadian journal of microbiology Volume: 52 ISSN: 0008-4166 ISO Abbreviation: Can. J. Microbiol. Publication Date: 2006 Dec |
Date Detail:
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Created Date: 2007-05-02 Completed Date: 2007-08-13 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0372707 Medline TA: Can J Microbiol Country: Canada |
Other Details:
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Languages: eng Pagination: 1199-207 Citation Subset: IM |
Affiliation:
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Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, PR, Brazil. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AF110271 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Bacterial Proteins / genetics*, metabolism Base Sequence Electrophoretic Mobility Shift Assay Gene Expression Regulation, Bacterial / drug effects Herbaspirillum / drug effects, genetics*, metabolism Integration Host Factors / genetics, metabolism Lac Operon Molecular Sequence Data Oxygen / pharmacology Promoter Regions, Genetic Protein Binding Quaternary Ammonium Compounds / pharmacology RNA Polymerase Sigma 54 / genetics*, metabolism Sequence Analysis, DNA Transcription Factors / genetics*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Integration Host Factors; 0/NifA protein, Bacteria; 0/NifB protein, Bacteria; 0/Quaternary Ammonium Compounds; 0/Transcription Factors; 7782-44-7/Oxygen; EC 2.7.7.6/RNA Polymerase Sigma 54 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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