Document Detail

The expression of nifB gene from Herbaspirillum seropedicae is dependent upon the NifA and RpoN proteins.
MedLine Citation:
PMID:  17473889     Owner:  NLM     Status:  MEDLINE    
The putative nifB promoter region of Herbaspirillum seropedicae contained two sequences homologous to NifA-binding site and a -24/-12 type promoter. A nifB::lacZ fusion was assayed in the backgrounds of both Escherichia coli and H. seropedicae. In E. coli, the expression of nifB::lacZ occurred only in the presence of functional rpoN and Klebsiella pneumoniae nifA genes. In addition, the integration host factor (IHF) stimulated the expression of the nifB::lacZ fusion in this background. In H. seropedicae, nifB expression occurred only in the absence of ammonium and under low levels of oxygen, and it was shown to be strictly dependent on NifA. DNA band shift experiments showed that purified K. pneumoniae RpoN and E. coli IHF proteins were capable of binding to the nifB promoter region, and in vivo dimethylsulfate footprinting showed that NifA binds to both NifA-binding sites. These results strongly suggest that the expression of the nifB promoter of H. seropedicae is dependent on the NifA and RpoN proteins and that the IHF protein stimulates NifA activation of nifB promoter.
Fabiane G M Rego; Fábio O Pedrosa; Leda S Chubatsu; M Geoffrey Yates; Roseli Wassem; Maria B R Steffens; Liu U Rigo; Emanuel M Souza
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Canadian journal of microbiology     Volume:  52     ISSN:  0008-4166     ISO Abbreviation:  Can. J. Microbiol.     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2007-05-02     Completed Date:  2007-08-13     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372707     Medline TA:  Can J Microbiol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  1199-207     Citation Subset:  IM    
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, PR, Brazil.
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MeSH Terms
Amino Acid Sequence
Bacterial Proteins / genetics*,  metabolism
Base Sequence
Electrophoretic Mobility Shift Assay
Gene Expression Regulation, Bacterial / drug effects
Herbaspirillum / drug effects,  genetics*,  metabolism
Integration Host Factors / genetics,  metabolism
Lac Operon
Molecular Sequence Data
Oxygen / pharmacology
Promoter Regions, Genetic
Protein Binding
Quaternary Ammonium Compounds / pharmacology
RNA Polymerase Sigma 54 / genetics*,  metabolism
Sequence Analysis, DNA
Transcription Factors / genetics*,  metabolism
Reg. No./Substance:
0/Bacterial Proteins; 0/Integration Host Factors; 0/NifA protein, Bacteria; 0/NifB protein, Bacteria; 0/Quaternary Ammonium Compounds; 0/Transcription Factors; 7782-44-7/Oxygen; EC Polymerase Sigma 54

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