| The expression and localization of membrane type-1 matrix metalloproteinase in human abdominal aortic aneurysms. | |
| | |
MedLine Citation:
|
PMID: 11496285 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND AND OBJECTIVE: Matrix metalloproteinase-2 (MMP-2) degrades both fibrillar collagens and elastin. MMP-2 is secreted as a latent 72-kd proenzyme that must be proteolytically processed to the 62-kd active form. In our laboratory we demonstrated a significant increase of active, matrix-bound MMP-2 in abdominal aortic aneurysmal (AAA) tissue compared with nonaneurysmal aorta with arteriosclerotic occlusive disease and normal aortic tissue. This increase in active MMP-2 is considered to be important in aneurysm pathogenesis, but the mechanism of its activation in aortic tissue is unknown. Membrane type-1 MMP (MT-1 MMP) is known to be an activator of MMP-2. The purpose of this study was to determine MT-1 MMP expression and its involvement in pro-MMP-2 activation in human aneurysmal tissue. METHODS: Infrarenal aortic tissue was obtained during the surgical repair of AAAs or the bypass of aortoiliac occlusive disease, or from nondiseased aorta, and the expression of MT-1 MMP messenger RNA was determined with Northern blot analysis. MT-1 MMP protein was determined with immunoblot and immunohistochemistry. The ability of aortic tissue to activate pro-MMP-2 was analyzed by incubating aortic tissue with exogenous radiolabeled pro-MMP-2. RESULTS: MT-1 MMP messenger RNA and protein are increased in AAA (P <.05) compared with arteriosclerotic occlusive disease and normal aortic tissue. Immunohistochemical analysis localized MT-1 MMP to aortic smooth muscle cells and macrophages in aneurysmal tissue. AAA tissue demonstrated a greater capacity to activate exogenous pro-MMP-2 compared with atherosclerotic and normal aortic tissue (P <.05). CONCLUSION: These studies demonstrate that MT-1 MMP is increased in AAA tissue and suggest that it may be important in AAA pathogenesis through its ability to activate pro-MMP-2 |
| | |
Authors:
|
A Nollendorfs; T C Greiner; H Nagase; B T Baxter |
Related Documents
:
|
14612975 - Immunohistochemical expression of metalloproteinases mmp-2 and mmp-9 in abdominal aorti... 15276585 - Antegrade endovascular repair of a coarctation-associated aneurysm through an upper hem... 12389235 - Does the presence of an iliac aneurysm affect outcome of endoluminal aaa repair? an ana... 15115155 - Computed tomographic angiography of endovascular abdominal aortic stent-grafts. 2944685 - Laser-assisted percutaneous angioplasty: initial clinical experience in peripheral arte... 21169145 - Rare variation in partial anomalous venous drainage in 2 cases: diagnosis, assessment m... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Journal of vascular surgery Volume: 34 ISSN: 0741-5214 ISO Abbreviation: J. Vasc. Surg. Publication Date: 2001 Aug |
Date Detail:
|
Created Date: 2001-08-09 Completed Date: 2001-10-04 Revised Date: 2012-10-03 |
Medline Journal Info:
|
Nlm Unique ID: 8407742 Medline TA: J Vasc Surg Country: United States |
Other Details:
|
Languages: eng Pagination: 316-22 Citation Subset: IM |
Affiliation:
|
Department of Surgery, University of Nebraska Medical Center, Omaha 68198-7690, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Aorta, Abdominal
/
cytology Aortic Aneurysm, Abdominal / metabolism* Cells, Cultured Humans Matrix Metalloproteinase 1 / biosynthesis*, genetics Matrix Metalloproteinase 2 / physiology* RNA, Messenger / analysis |
| Grant Support | |
ID/Acronym/Agency:
|
1RO1HL62400-01A1/HL/NHLBI NIH HHS; AR39189/AR/NIAMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/RNA, Messenger; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.7/Matrix Metalloproteinase 1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Simple geometric characteristics fail to reliably predict abdominal aortic aneurysm wall stresses.
Next Document: Acute arterial thrombosis causes endothelial dysfunction: a new paradigm for thrombolytic therapy.