Document Detail

The expression of a grapefruit gene encoding an isoflavone reductase-like protein is induced in response to UV irradiation.
MedLine Citation:
PMID:  9520276     Owner:  NLM     Status:  MEDLINE    
Exposure of harvested grapefruit to UV-C (254 nm) irradiation was previously found to induce resistance against the green mold decay caused by Penicillium digitatum. In order to gain insight into the mechanism of this UV-induced resistance we initiated a study for isolation of genes induced during this process. Using the differential display method we cloned cDNA representing an mRNA which is accumulated in grapefruit peel upon UV irradiation. Sequence analysis revealed that this cDNA represents a gene encoding for an isoflavone reductase-like protein and was termed IRL (isoflavone reductase-like). The grapefruit IRL protein sequence has high homology also to a novel family of other isoflavone reductase-like proteins present in few non-legume plants and whose function is not clear yet. The UV dose, and time following it, which lead to maximal accumulation of the IRL transcript were found to be similar to those leading to maximal induced resistance. The expression of the IRL gene was demonstrated to be induced also by wounding and pathogen infection.
A Lers; S Burd; E Lomaniec; S Droby; E Chalutz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Plant molecular biology     Volume:  36     ISSN:  0167-4412     ISO Abbreviation:  Plant Mol. Biol.     Publication Date:  1998 Apr 
Date Detail:
Created Date:  1998-04-09     Completed Date:  1998-04-09     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9106343     Medline TA:  Plant Mol Biol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  847-56     Citation Subset:  IM    
Department of Postharvest Science of Fresh Produce, The Volcani Center, Bet Dagan, Israel.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Blotting, Southern
Carrier Proteins / genetics*
Citrus / enzymology,  genetics*,  immunology,  radiation effects
Cloning, Molecular
DNA, Complementary
DNA, Plant / radiation effects
Gene Expression Regulation, Enzymologic / radiation effects*
Gene Expression Regulation, Plant / radiation effects*
Immunity, Innate / radiation effects
Molecular Sequence Data
Oxidoreductases / genetics*
Plant Proteins / genetics*
Sequence Alignment
Ultraviolet Rays*
Reg. No./Substance:
0/Carrier Proteins; 0/DNA, Complementary; 0/DNA, Plant; 0/IRL protein, Citrus x paradisi; 0/Plant Proteins; EC 1.-/Oxidoreductases

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