Document Detail

The expression of basic fibroblast growth factor and its receptor in cell lines derived from normal human mammary gland and a benign mammary lesion.
MedLine Citation:
PMID:  8270619     Owner:  NLM     Status:  MEDLINE    
mRNA for basic Fibroblast Growth Factor (bFGF) was expressed in a series of SV40-transformed human mammary cell lines as molecules of 7.1, 3.6, 2.0 and 1.2 kb. This expression was much weaker in those lines of epithelial morphology than in myoepithelial-like cell lines derived from them. It was confirmed, using northern hybridization to single-stranded RNA probes, that the multiple mRNAs were transcribed from the coding strand for bFGF. bFGF activity was detected in extracts of the cells and the relative amounts of activity corresponded in general to the amounts of mRNA found. Similar results were obtained from spontaneously transformed cell lines derived from a human benign breast lesion. The presence of bFGF protein in the extracts was confirmed by western blotting, which showed a band of 18-19 kDa, migrating in the same position as authentic bFGF; in addition, the myoepithelial-like cells showed prominent bands of bFGF at 24 and 26 kDa. No FGF receptor was detectable by the binding of 125I-bFGF to the SV40-transformed cell lines or to the epithelial cell lines from the benign breast lesion, but both high- and low-affinity receptors were found on myoepithelial-like cells derived from the latter. The results indicate that differentiation to the human myoepithelial-like phenotype in culture is associated with the enhanced expression of bFGF, and it is suggested that bFGF, immunocytochemically detected in the basement membrane of the human breast, may arise, at least in part, from the myoepithelial cells of the mammary parenchyma.
Y Ke; D G Fernig; M C Wilkinson; J H Winstanley; J A Smith; P S Rudland; R Barraclough
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cell science     Volume:  106 ( Pt 1)     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  1993 Sep 
Date Detail:
Created Date:  1994-01-31     Completed Date:  1994-01-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  135-43     Citation Subset:  IM    
Department of Biochemistry, University of Liverpool, UK.
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MeSH Terms
Breast / cytology,  metabolism*
Cell Line, Transformed
Epithelial Cells
Epithelium / metabolism
Fibroblast Growth Factor 2 / biosynthesis*
Fibrocystic Breast Disease / metabolism*,  pathology
Gene Expression Regulation
RNA, Messenger / analysis
Receptors, Fibroblast Growth Factor / biosynthesis*
Reg. No./Substance:
0/RNA, Messenger; 0/Receptors, Fibroblast Growth Factor; 103107-01-3/Fibroblast Growth Factor 2

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