Document Detail

The expression of apoptosis-related proteins Bcl-2 and Ki67 in endometrium of ovulatory menstrual cycles.
MedLine Citation:
PMID:  12186988     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: During the menstrual cycle, a rapid sequence of proliferation, differentiation and cell death occurs in the human endometrium. Mechanisms involved in cell proliferation have been studied extensively. Apoptosis has recently been recognized to be a physiologic phenomenon. The aim of this study was to investigate the mechanisms involved in hormone-dependent tissue remodeling by measuring Bcl-2, an apoptosis inhibitor, and Ki67, a proliferation marker, as expressed in normal human endometrium. METHODS: Paraffin-embedded endometrial sections of 30 uteri were immunostained for Bcl-2 and Ki67; expression was scored in cavitary epithelium, functional and basal glandular epithelial and stromal cells of the endometrium. RESULTS: Bcl-2 expression increased in the proliferative phases and decreased significantly in the secretory phases, especially in glandular epithelial cells (131 +/- 45 for functional laminal cells and 227 +/- 68 for basal laminal cells to 0). Ki67 expression showed the same cyclic pattern with a later onset (145 +/- 63 for functional laminal cells and 13 +/- 8 for basal laminal cells to 0). CONCLUSION: Bcl-2 promotes cell survival by preventing apoptosis. Proliferation is the result of increasing estradiol concentration, high estrogen receptor expression and growth hormones and high Bcl-2 and Ki67 expression. After the onset of progesterone production, Bcl-2 levels decrease and Ki67 levels and androgen receptor expression in stromal cells disappear resulting in cell disintegration and menstruation. Persistent Bcl-2 expression, like we saw in basal laminal stromal and epithelial cells, accounts for the privilege of escaping from apoptosis-inducing signals. This allows reconstruction of the functional endometrium from its preserved basal layer after menstruation.
Helena J M M Mertens; Maas J Heineman; Johannes L H Evers
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Gynecologic and obstetric investigation     Volume:  53     ISSN:  0378-7346     ISO Abbreviation:  Gynecol. Obstet. Invest.     Publication Date:  2002  
Date Detail:
Created Date:  2002-08-20     Completed Date:  2003-02-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7900587     Medline TA:  Gynecol Obstet Invest     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  224-30     Citation Subset:  IM    
Copyright Information:
Copyright 2002 S. Karger AG, Basel
Department of Obstetrics and Gynecology, Research Institute Growth and Development, GROW, University Hospital Maastricht, The Netherlands.
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MeSH Terms
Biological Markers / analysis
Biopsy, Needle
Cell Division
Cohort Studies
Endometrium / metabolism*,  physiology
Ki-67 Antigen / biosynthesis*
Menstrual Cycle / physiology*
Middle Aged
Prospective Studies
Proto-Oncogene Proteins c-bcl-2 / biosynthesis*
Reference Values
Regeneration / physiology*
Sensitivity and Specificity
Statistics, Nonparametric
Reg. No./Substance:
0/Biological Markers; 0/Ki-67 Antigen; 0/Proto-Oncogene Proteins c-bcl-2

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