| The expression of apoptosis-related proteins Bcl-2 and Ki67 in endometrium of ovulatory menstrual cycles. | |
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MedLine Citation:
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PMID: 12186988 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: During the menstrual cycle, a rapid sequence of proliferation, differentiation and cell death occurs in the human endometrium. Mechanisms involved in cell proliferation have been studied extensively. Apoptosis has recently been recognized to be a physiologic phenomenon. The aim of this study was to investigate the mechanisms involved in hormone-dependent tissue remodeling by measuring Bcl-2, an apoptosis inhibitor, and Ki67, a proliferation marker, as expressed in normal human endometrium. METHODS: Paraffin-embedded endometrial sections of 30 uteri were immunostained for Bcl-2 and Ki67; expression was scored in cavitary epithelium, functional and basal glandular epithelial and stromal cells of the endometrium. RESULTS: Bcl-2 expression increased in the proliferative phases and decreased significantly in the secretory phases, especially in glandular epithelial cells (131 +/- 45 for functional laminal cells and 227 +/- 68 for basal laminal cells to 0). Ki67 expression showed the same cyclic pattern with a later onset (145 +/- 63 for functional laminal cells and 13 +/- 8 for basal laminal cells to 0). CONCLUSION: Bcl-2 promotes cell survival by preventing apoptosis. Proliferation is the result of increasing estradiol concentration, high estrogen receptor expression and growth hormones and high Bcl-2 and Ki67 expression. After the onset of progesterone production, Bcl-2 levels decrease and Ki67 levels and androgen receptor expression in stromal cells disappear resulting in cell disintegration and menstruation. Persistent Bcl-2 expression, like we saw in basal laminal stromal and epithelial cells, accounts for the privilege of escaping from apoptosis-inducing signals. This allows reconstruction of the functional endometrium from its preserved basal layer after menstruation. |
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Authors:
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Helena J M M Mertens; Maas J Heineman; Johannes L H Evers |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Gynecologic and obstetric investigation Volume: 53 ISSN: 0378-7346 ISO Abbreviation: Gynecol. Obstet. Invest. Publication Date: 2002 |
Date Detail:
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Created Date: 2002-08-20 Completed Date: 2003-02-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7900587 Medline TA: Gynecol Obstet Invest Country: Switzerland |
Other Details:
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Languages: eng Pagination: 224-30 Citation Subset: IM |
Copyright Information:
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Copyright 2002 S. Karger AG, Basel |
Affiliation:
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Department of Obstetrics and Gynecology, Research Institute Growth and Development, GROW, University Hospital Maastricht, The Netherlands. hmertens@orbisconcern.nl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Apoptosis* Biological Markers / analysis Biopsy, Needle Cell Division Cohort Studies Endometrium / metabolism*, physiology Female Humans Immunohistochemistry Ki-67 Antigen / biosynthesis* Menstrual Cycle / physiology* Middle Aged Ovulation Probability Prospective Studies Proto-Oncogene Proteins c-bcl-2 / biosynthesis* Reference Values Regeneration / physiology* Sensitivity and Specificity Statistics, Nonparametric |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Ki-67 Antigen; 0/Proto-Oncogene Proteins c-bcl-2 |
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