Document Detail


The expression of apoptosis related genes in the first trimester human placenta using a short term in vitro model.
MedLine Citation:
PMID:  15711929     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Using in vitro model for studying the induction and inhibition of spontaneous apoptosis in human first trimester placental villi, mediated by the free radical scavenger SOD, we have examined the expression of bcl-xL, bax, Caspase-3 and PARP (Poly ADP-ribosyl). An increase in apoptosis was associated with activation of PARP and an increase and activation of Caspase-3. There was no significant change in bcl-x or bax. Therefore bcl-x and bax do not appear to have a significant role in apoptosis in the first trimester in vitro. Cleavage of Caspase-3 rather than transcriptional regulation appears to be the main determinant of Caspase-3 activity in first trimester placental villi.
Authors:
A K Charles; S Hisheh; D Liu; R M Rao; B J Waddell; J E Dickinson; A J Rao; A M Dharmarajan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Apoptosis : an international journal on programmed cell death     Volume:  10     ISSN:  1360-8185     ISO Abbreviation:  Apoptosis     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2005-02-15     Completed Date:  2005-06-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9712129     Medline TA:  Apoptosis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  135-40     Citation Subset:  IM    
Affiliation:
Department of Pathology King Edward Memorial Hospital, University of Western Australia, Perth, Western Australia. adrian.charles@health.wa.gov.aau
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / genetics*
Caspase 3
Caspases / metabolism
Cell Culture Techniques*
Chorionic Villi / physiology*
Female
Free Radical Scavengers / metabolism
Humans
Placenta*
Pregnancy
Pregnancy Trimester, First*
RNA, Messenger / analysis
Superoxide Dismutase / metabolism
Chemical
Reg. No./Substance:
0/Free Radical Scavengers; 0/RNA, Messenger; EC 1.15.1.1/Superoxide Dismutase; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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