| The expression of TGF-β3 for epithelial-mesenchyme transdifferentiated MEE in palatogenesis. | |
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MedLine Citation:
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PMID: 20967564 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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The fate of the palatal medial edge epithelial (MEE) cells undergoes programming cell death, migration, and epithelial-mesenchymal transdifferentiation (EMT) coincident with the process of palatal fusion and disappearance of MEE. Mesenchymal cells in the palate have both cranial neural crest (CNC) and non-CNC origins. The objectives of this study were to identify the populations of palatal mesenchymal cells using β-galactosidase (β-gal) and DiI cell lineage markers, and to determine whether MEE-derived cells continued to express transforming growth factor-β3 (TGF-β3) and transforming growth factor-β type III receptor (TβR-III), which were specific for MEE. A model has been developed using Wnt1 tissue specific expression of Cre-recombinase to activate β-gal solely in the CNC. The expressions of TGF-β3 and TβR-III in MEE were temporally correlated with critical events in palatogenesis. Three cell populations could be distinguished in the palatal mesenchymal CNC-derived, non-CNC derived and MEE-derived. After fusion, β-gal⁻ and DiI+ mesenchymal cells continued to express TGF-β3, however TβR-III was expressed only in the epithelial MEE, as well as keratin expression. In addition, we performed laser capture microdissection to identify mRNA expression of isolated DiI+ MEE cells. Both epithelial and transdifferentiated MEE have expressed TGF-β3, however, TβR-III was only expressed in epithelium. Extracellular matrix, especially MMP13 has been expressed coincident with fused stage which can be strongly associated with TGF-β3. These results demonstrate that combining a heritable marker and a cell lineage dye can distinguish different populations of mesenchymal cells in the developing palate. Furthermore, TGF-β3 and MMP13 could be strongly associated with EMT in palatogenesis. |
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Authors:
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Akira Nakajima; Eiji Tanaka; Yoshihiro Ito; Masao Maeno; Koichi Iwata; Noriyoshi Shimizu; Charles F Shuler |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-10-22 |
Journal Detail:
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Title: Journal of molecular histology Volume: 41 ISSN: 1567-2387 ISO Abbreviation: J. Mol. Histol. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-08 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101193653 Medline TA: J Mol Histol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 343-55 Citation Subset: IM |
Affiliation:
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Department of Orthodontics, Nihon University School of Dentistry, 1-8-13 Kanda Surugadai, Chiyoda-ku, Tokyo 1018314, Japan. nakajima-a@dent.nihon-u.ac.jp |
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Descriptor/Qualifier:
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| Grant Support | |
ID/Acronym/Agency:
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P01DE-12941/DE/NIDCR NIH HHS; R01DE-12711/DE/NIDCR NIH HHS |
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