Document Detail


The expression of PLK-1 in cervical carcinoma: a possible target for enhancing chemosensitivity.
MedLine Citation:
PMID:  19775446     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Polo-like kinase-1 (PLK-1) is reported to be upregulated in a variety of human tumors and is implicated in cell proliferation and survival. However, its importance in cervical carcinoma has not yet been fully elucidated.
METHODS: We examined PLK-1 expression in cervical carcinoma tissues using immunohistochemical staining. Furthermore, we blocked PLK-1 expression in HeLa cells using specific siRNA and detected the cell cycle, cell proliferation and chemosensitivity using western blotting, MTT and flow cytometry.
RESULTS: We provide evidence that expression of PLK-1 exists in human cervical carcinoma tissues and establish an association with tumor size. Furthermore, we show that PLK-1 knockdown by transfection of siRNA induces accumulation of HeLa cells in the G2/M cell cycle phase and enhances cisplatin-induced apoptosis.
CONCLUSION: Our results indicate that PLK-1 production in HeLa cells might be critical in determining whether cells survive or undergo apoptosis. Therefore, targeting PLK-1 might be a promising strategy for enhancing sensitivity to chemotherapeutic reagents in cervical carcinoma.
Authors:
Yuan Zhang; Yu Liu; Yuan-Xian Yang; Jia-Hong Xia; Hong-Xiu Zhang; Hua-Bin Li; Chun-Zhao Yu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-23
Journal Detail:
Title:  Journal of experimental & clinical cancer research : CR     Volume:  28     ISSN:  1756-9966     ISO Abbreviation:  J. Exp. Clin. Cancer Res.     Publication Date:  2009  
Date Detail:
Created Date:  2009-10-14     Completed Date:  2009-11-24     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  8308647     Medline TA:  J Exp Clin Cancer Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  130     Citation Subset:  IM    
Affiliation:
Department of Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei, PR China. yuanzhang75@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / physiology
Blotting, Western
Caspase 3 / metabolism
Cell Cycle Proteins / biosynthesis*
Drug Resistance, Neoplasm / physiology*
Female
Flow Cytometry
HeLa Cells
Humans
Immunohistochemistry
Protein-Serine-Threonine Kinases / biosynthesis*
Proto-Oncogene Proteins / biosynthesis*
RNA, Messenger
RNA, Small Interfering
Reverse Transcriptase Polymerase Chain Reaction
Tumor Markers, Biological / analysis*
Uterine Cervical Neoplasms / enzymology*
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Proto-Oncogene Proteins; 0/RNA, Messenger; 0/RNA, Small Interfering; 0/Tumor Markers, Biological; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/polo-like kinase 1; EC 3.4.22.-/Caspase 3
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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