Document Detail


The expression of PDGF alpha- and beta-receptors in subpopulations of PDGF-producing cells implicates autocrine stimulatory loops in the control of proliferation in cytotrophoblasts that have invaded the maternal endometrium.
MedLine Citation:
PMID:  1389228     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to explain the high proliferative potential of human placental cytotrophoblasts, we have addressed the potential involvement of platelet-derived growth factor (PDGF) ligand and receptors. Although PDGF is usually described as a mitogen for cells of mesenchymal origin, we show in this report that extra-villous term placental cytotrophoblasts express the PDGF alpha- and beta-receptor genes, both in vivo and in vitro. In addition, cytotrophoblasts produce significant amounts of PDGF-B protein. By immunohistochemical analysis of receptor expression, we found that the PDGF alpha-receptors could be detected at the cell surface, while the PDGF beta-receptors were only detected intracellularly. In addition, double immunostaining analysis showed that the PDGF alpha- and beta-receptor molecules are expressed in different subpopulations of cytotrophoblasts. The addition of PDGF-AA and PDGF-BB homodimers to cytotrophoblast primary cultures induced a significant increase in DNA synthesis. We conclude, therefore, that PDGF is a growth factor for placental cytotrophoblasts and suggest that the growth of cytotrophoblasts can partly be explained by a PDGF autostimulatory loop, limited by the number of receptor-positive cytotrophoblasts.
Authors:
L Holmgren; L Claesson-Welsh; C H Heldin; R Ohlsson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Growth factors (Chur, Switzerland)     Volume:  6     ISSN:  0897-7194     ISO Abbreviation:  Growth Factors     Publication Date:  1992  
Date Detail:
Created Date:  1992-10-26     Completed Date:  1992-10-26     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9000468     Medline TA:  Growth Factors     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  219-31     Citation Subset:  IM    
Affiliation:
Laboratory for Molecular Development and Tumour Biology, Institute of Experimental Drug Research, Karolinska Hospital, Stockholm, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Cell Membrane / chemistry
Cells, Cultured
Endometrium / cytology
Female
Gene Expression / physiology*
Humans
Placenta / chemistry*,  cytology
Platelet-Derived Growth Factor / physiology*
Pregnancy
Receptors, Platelet-Derived Growth Factor / analysis*,  genetics
Trophoblasts / chemistry
Chemical
Reg. No./Substance:
0/Platelet-Derived Growth Factor; EC 2.7.10.1/Receptors, Platelet-Derived Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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