Document Detail


An experimental system to study responses of Medicago truncatula roots to chitin oligomers of high degree of polymerization and other microbial elicitors.
MedLine Citation:
PMID:  23314495     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A fully acetylated, soluble CO preparation of mean DP of ca. 7 was perceived with high sensitivity by M. truncatula in a newly designed versatile root elicitation assay. The root system of legume plants interacts with a large variety of microorganisms, either pathogenic or symbiotic. Understanding how legumes recognize and respond specifically to pathogen-associated or symbiotic signals requires the development of standardized bioassays using well-defined preparations of the corresponding signals. Here we describe the preparation of chitin oligosaccharide (CO) fractions from commercial chitin and their characterization by a combination of liquid-state and solid-state nuclear magnetic resonance spectroscopy. We show that the CO fraction with highest degree of polymerization (DP) became essentially insoluble after lyophilization. However, a fully soluble, fully acetylated fraction with a mean DP of ca. 7 was recovered and validated by showing its CERK1-dependent activity in Arabidopsis thaliana. In parallel, we developed a versatile root elicitation bioassay in the model legume Medicago truncatula, using a hydroponic culture system and the Phytophthora β-glucan elicitor as a control elicitor. We then showed that M. truncatula responded with high sensitivity to the CO elicitor, which caused the production of extracellular reactive oxygen species and the transient induction of a variety of defense-associated genes. In addition, the bioassay allowed detection of elicitor activity in culture filtrates of the oomycete Aphanomyces euteiches, opening the way to the analysis of recognition of this important legume root pathogen by M. truncatula.
Authors:
A Nars; T Rey; C Lafitte; S Vergnes; S Amatya; C Jacquet; B Dumas; C Thibaudeau; L Heux; A Bottin; J Fliegmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-01-13
Journal Detail:
Title:  Plant cell reports     Volume:  32     ISSN:  1432-203X     ISO Abbreviation:  Plant Cell Rep.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-15     Completed Date:  2013-09-18     Revised Date:  2013-11-07    
Medline Journal Info:
Nlm Unique ID:  9880970     Medline TA:  Plant Cell Rep     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  489-502     Citation Subset:  IM    
Affiliation:
Université de Toulouse, UPS, UMR5546, Laboratoire de Recherche en Sciences Végétales (LRSV), BP 42617, 31326, Castanet-Tolosan, France.
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MeSH Terms
Descriptor/Qualifier:
Acetylation
Aphanomyces
Arabidopsis / physiology
Arabidopsis Proteins / metabolism
Chitin / chemistry,  pharmacology*
Gene Expression Regulation, Plant
Magnetic Resonance Spectroscopy
Medicago truncatula / drug effects,  genetics,  physiology*
Phytophthora
Plant Diseases
Plant Roots / drug effects,  genetics,  physiology*
Polymerization
Protein-Serine-Threonine Kinases / metabolism
Reactive Oxygen Species / metabolism
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/Reactive Oxygen Species; 1398-61-4/Chitin; EC 2.7.11.1/CERK1 protein, Arabidopsis; EC 2.7.11.1/Protein-Serine-Threonine Kinases

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