| The expansion of human ES and iPS cells on porous membranes and proliferating human adipose-derived feeder cells. | |
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MedLine Citation:
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PMID: 20674000 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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For clinical application of human embryonic stem cells (hESCs), it is critical to develop hESC culture techniques that completely exclude the use of animal feeder cells, mitotic inhibition, and enzyme treatments used in conventional hESC culture systems. Toward this goal, we attempted to maintain hESCs and induced pluripotent stem (iPS) cells on porous membranes (PMs) with proliferative human adipose-derived stromal cells (ASCs) seeded on the bottom surface of inverted PMs. This culture condition will ensure that the two cell types are separate from each other, yet retain the ability to interact through the pores of the membrane. We found that hESCs and iPS cells can be maintained stably and mechanically transferred without the need for enzyme treatment. In addition, the pluripotency of hESCs and iPS cells was stably maintained, as evidenced by immunostaining of Oct4, SSEA3/4 and TRA-1-60 as well as RT-PCR analyses of Nanog, Oct4 and Sox2 expression. Furthermore, hESCs cultured on PMs showed a normal karyotype and in vivo teratoma formation containing all three germ layers. |
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Authors:
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Seung-Taeh Hwang; Sun-Woong Kang; Suk-Jun Lee; Tae-Hee Lee; Wonhee Suh; Sung Han Shim; Dong-Ryul Lee; Lakeshia J Taite; Kwang-Soo Kim; Soo-Hong Lee |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-31 |
Journal Detail:
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Title: Biomaterials Volume: 31 ISSN: 1878-5905 ISO Abbreviation: Biomaterials Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-08-30 Completed Date: 2010-12-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 8012-21 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Biomedical Science, CHA Stem Cell Institute, CHA University, Seoul, Republic of Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adipose Tissue
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cytology*,
ultrastructure Cell Proliferation Cells, Cultured Embryonic Stem Cells / cytology*, metabolism, ultrastructure Humans Immunohistochemistry Induced Pluripotent Stem Cells / cytology*, metabolism Karyotyping Membranes, Artificial* Porosity Stromal Cells / cytology Surface Properties Teratoma / pathology |
| Chemical | |
Reg. No./Substance:
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0/Membranes, Artificial |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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