| On the existence of two GABA pools associated with newly synthesized GABA and with newly taken up GABA in nerve terminals. | |
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MedLine Citation:
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PMID: 6136927 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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[14C]Glutamic acid and [3H]GABA were injected into the lateral ventricle of mouse and then [14C]GABA and [3H]GABA in synaptosomes isolated from the animals were analysed. The [14C]GABA was interpreted to be newly synthesized GABA from [14C]glutamic acid while the [3H]GABA to be newly taken up GABA. We have obtained the following results: (1) when the animals were pretreated with aminooxyacetic acid and thus the GABA content in synaptosomes increased to about 2 times of the control level, only the [3H]GABA was enhanced to 3 times of the control level without any change of [14C]GABA, (2) the release of [14C]GABA from synaptosomes by high K+ depolarization was 1.5 times greater than that of [3H]GABA, (3) the releases of both [14C]GABA and [3H]GABA were increased in the presence of cold GABA, L-2,4-diaminobutyric acid or gamma-amino-beta-hydroxybutyric acid, but only slightly increased in the presence of beta-alanine. These results would suggest that newly synthesized GABA and newly taken up GABA localized individually in different pools, which might localize either in different nerve terminals or separately in the same nerve terminal. |
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Authors:
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M Abe; M Matsuda |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Neurochemical research Volume: 8 ISSN: 0364-3190 ISO Abbreviation: Neurochem. Res. Publication Date: 1983 May |
Date Detail:
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Created Date: 1983-10-08 Completed Date: 1983-10-08 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7613461 Medline TA: Neurochem Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 563-73 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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metabolism Animals Brain / metabolism* Carbon Radioisotopes / diagnostic use Glutamates / metabolism Glutamic Acid Kinetics Mice Mice, Inbred Strains Synaptosomes / metabolism* Tritium / diagnostic use gamma-Aminobutyric Acid / biosynthesis, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Carbon Radioisotopes; 0/Glutamates; 10028-17-8/Tritium; 56-12-2/gamma-Aminobutyric Acid; 56-86-0/Glutamic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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