Document Detail


On the existence of two GABA pools associated with newly synthesized GABA and with newly taken up GABA in nerve terminals.
MedLine Citation:
PMID:  6136927     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
[14C]Glutamic acid and [3H]GABA were injected into the lateral ventricle of mouse and then [14C]GABA and [3H]GABA in synaptosomes isolated from the animals were analysed. The [14C]GABA was interpreted to be newly synthesized GABA from [14C]glutamic acid while the [3H]GABA to be newly taken up GABA. We have obtained the following results: (1) when the animals were pretreated with aminooxyacetic acid and thus the GABA content in synaptosomes increased to about 2 times of the control level, only the [3H]GABA was enhanced to 3 times of the control level without any change of [14C]GABA, (2) the release of [14C]GABA from synaptosomes by high K+ depolarization was 1.5 times greater than that of [3H]GABA, (3) the releases of both [14C]GABA and [3H]GABA were increased in the presence of cold GABA, L-2,4-diaminobutyric acid or gamma-amino-beta-hydroxybutyric acid, but only slightly increased in the presence of beta-alanine. These results would suggest that newly synthesized GABA and newly taken up GABA localized individually in different pools, which might localize either in different nerve terminals or separately in the same nerve terminal.
Authors:
M Abe; M Matsuda
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Neurochemical research     Volume:  8     ISSN:  0364-3190     ISO Abbreviation:  Neurochem. Res.     Publication Date:  1983 May 
Date Detail:
Created Date:  1983-10-08     Completed Date:  1983-10-08     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  563-73     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism
Animals
Brain / metabolism*
Carbon Radioisotopes / diagnostic use
Glutamates / metabolism
Glutamic Acid
Kinetics
Mice
Mice, Inbred Strains
Synaptosomes / metabolism*
Tritium / diagnostic use
gamma-Aminobutyric Acid / biosynthesis,  metabolism*
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Carbon Radioisotopes; 0/Glutamates; 10028-17-8/Tritium; 56-12-2/gamma-Aminobutyric Acid; 56-86-0/Glutamic Acid

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