Document Detail


HAG2/MYB76 and HAG3/MYB29 exert a specific and coordinated control on the regulation of aliphatic glucosinolate biosynthesis in Arabidopsis thaliana.
MedLine Citation:
PMID:  18042203     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In a previous transactivation screen, two Arabidopsis thaliana R2R3-MYB transcription factors, HAG2/MYB76 and HAG3/MYB29, along with the already characterized HAG1/MYB28, were identified as putative regulators of aliphatic glucosinolate biosynthesis. Molecular and biochemical characterization of HAG2/MYB76 and HAG3/MYB29 functions was performed using transformants with increased or repressed transcript levels. Real-time PCR assays, cotransformation assays and measurements of glucosinolate contents were used to assess the impact of both MYB factors on the steady-state level of glucosinolate biosynthetic genes and accumulation of aliphatic glucosinolates. Both HAG2/MYB76 and HAG3/MYB29 were shown to be positive regulators of aliphatic glucosinolate biosynthesis. Expression of promoter-beta-glucuronidase (GUS) fusions indicated GUS activities in both vegetative and generative organs, with distinct characteristics for each MYB factor. HAG1/MYB28, HAG2/MYB76 and HAG3/MYB29 reciprocally transactivated each other in the control of aliphatic glucosinolate biosynthesis and downregulated the expression of genes involved in the control of indolic glucosinolate biosynthesis, pointing to a reciprocal negative regulation of these two pathways. All three HAG transcription factors exert a coordinated control on aliphatic glucosinolate biosynthesis.
Authors:
Tamara Gigolashvili; Martin Engqvist; Ruslan Yatusevich; Caroline M?ller; Ulf-Ingo Fl?gge
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-11-27
Journal Detail:
Title:  The New phytologist     Volume:  177     ISSN:  0028-646X     ISO Abbreviation:  New Phytol.     Publication Date:  2008  
Date Detail:
Created Date:  2008-01-23     Completed Date:  2008-05-20     Revised Date:  2010-05-20    
Medline Journal Info:
Nlm Unique ID:  9882884     Medline TA:  New Phytol     Country:  England    
Other Details:
Languages:  eng     Pagination:  627-42     Citation Subset:  IM    
Affiliation:
Botanisches Institut der Universit?t zu K?ln, Gyrhofstrasse 15, D-50931 K?ln, Germany.
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MeSH Terms
Descriptor/Qualifier:
Acetic Acids / metabolism
Adaptation, Physiological*
Arabidopsis / genetics,  metabolism*
Arabidopsis Proteins / genetics,  metabolism*
Cyclopentanes / metabolism
Gene Expression Regulation, Plant
Glucosinolates / biosynthesis*
Mutagenesis, Insertional
Oxylipins / metabolism
Phenotype
Transcription Factors / genetics,  metabolism*
Transcriptional Activation
Chemical
Reg. No./Substance:
0/Acetic Acids; 0/Arabidopsis Proteins; 0/Cyclopentanes; 0/Glucosinolates; 0/MYB76 protein, Arabidopsis; 0/Myb29 protein, Arabidopsis; 0/Oxylipins; 0/Transcription Factors; 1211-29-6/methyl jasmonate; EC 2.3.1.48/GCN5 protein, Arabidopsis

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