Document Detail

The evolution of clot-promoting and amidolytic activities in mixtures of Hageman factor (factor XII) and ellagic acid.
MedLine Citation:
PMID:  7097109     Owner:  NLM     Status:  MEDLINE    
Ellagic acid (4,4',5,5',6,6'-hexahydroxydiphenic acid 2,6,2',6'-dilactone) can substitute for negatively charged surfaces as a stimulus to reactions of the intrinsic pathway. Incubation of solutions of 4 X 10(-6)M ellagic acid with purified HF (factor XII) induced clot-promoting and amidolytic activity. Clot-promoting activity tested on a substrate of HF-deficient plasma evolved much more rapidly than amidolytic activity. Clot-promoting activity generated in mixtures of HF and ellagic acid alone, but amidolytic activity was observed only if additional proteins such as albumin were also present. Treatment of purified HF with DFP or filtration of HF through columns of SBTI bound to agarose did not prevent its subsequent activation by ellagic acid. Solutions of SBTI, at high concentration, partly inhibited the generation of amidolytic activity,wheras popcorn inhibitor and a crude IgG fraction of anti-HF inhibited the amidolytic activity that had been generated in a mixture of HF and ellagic acid. Generation of clotting and amidolytic properties was accompanied by scission of HF within an internal disulfide loop and by cleavage of HF into fragments with approximate MWs of 50,000 and 30,000; cleavage was completely blocked by popcorn inhibitor and partially blocked by high concentrations of SBTI. These experiments demonstrate that ellagic acid can activate HF in a manner analogous to negatively charged solids such as glass or kaolin.
O D Ratnoff; H Saito
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of laboratory and clinical medicine     Volume:  100     ISSN:  0022-2143     ISO Abbreviation:  J. Lab. Clin. Med.     Publication Date:  1982 Aug 
Date Detail:
Created Date:  1982-09-10     Completed Date:  1982-09-10     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375375     Medline TA:  J Lab Clin Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  248-60     Citation Subset:  AIM; IM    
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MeSH Terms
Amides / blood*
Benzopyrans / pharmacology*
Blood Coagulation / drug effects*
Blood Proteins / metabolism*
Ellagic Acid / pharmacology*
Factor XII / metabolism*
Isoflurophate / pharmacology
Serum Albumin, Bovine / metabolism
Trypsin Inhibitors / pharmacology
Grant Support
Reg. No./Substance:
0/Amides; 0/Benzopyrans; 0/Blood Proteins; 0/Serum Albumin, Bovine; 0/Trypsin Inhibitors; 476-66-4/Ellagic Acid; 55-91-4/Isoflurophate; 9001-30-3/Factor XII

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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