Document Detail


The evolution of cardenolide-resistant forms of Na(+) ,K(+) -ATPase in Danainae butterflies.
MedLine Citation:
PMID:  22126595     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Cardenolides are a class of plant secondary compounds that inhibit the proper functioning of the Na(+) , K(+) -ATPase enzyme in susceptible animals. Nonetheless, many insect species are able to sequester cardenolides for their own defence. These include butterflies in the subfamily Danainae (Family: Nymphalidae) such as the monarch (Danaus plexippus). Previous studies demonstrated that monarchs harbour an asparagine (N) to histidine (H) substitution (N122H) in the α subunit of Na(+) , K(+) -ATPase (ATPα) that reduces this enzyme's sensitivity to cardenolides. More recently, it has been suggested that at ATPα position 111, monarchs may also harbour a leucine (L)/glutamine (Q) polymorphism. This later amino acid could also contribute to cardenolide insensitivity. However, here we find that incorrect annotation of the initially reported DNA sequence for ATPα has led to several erroneous conclusions. Using a population genetic and phylogenetic analysis of monarchs and their close relatives, we show that an ancient Q111L substitution occurred prior to the radiation of all Danainae, followed by a second substitution at the same site to valine (V), which arose before the diversification of the Danaus genus. In contrast, N122H appears to be a recent substitution specific to monarchs. Surprisingly, examination of a broader insect phylogeny reveals that the same progression of amino acid substitutions (Q111L → L111V + N122H) has also occurred in Chyrsochus beetles (Family: Chrysomelidae, Subfamily: Eumolpinae) that feed on cardenolide-containing host plants. The parallel pattern of amino acid substitution in these two distantly related lineages is consistent with an adaptive role for these substitutions in reducing cardenolide sensitivity and suggests that their temporal order may be limited by epistatic interactions.
Authors:
Matthew L Aardema; Ying Zhen; Peter Andolfatto
Related Documents :
20625935 - The complex and important cellular and metabolic functions of saturated fatty acids.
14753605 - Focal fatty sparing of the pancreatic head in cystic fibrosis: ct findings.
25329915 - Carbon and nitrogen isotopes from top predator amino acids reveal rapidly shifting ocea...
19323735 - Formation of trans fatty acids in ground beef and frankfurters due to irradiation.
101375 - The phenoloxidases of the ascomycete podospora anserina. structural differences between...
9357925 - Medium chain 3-ketoacyl-coenzyme a thiolase deficiency: a new disorder of mitochondrial...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-11-29
Journal Detail:
Title:  Molecular ecology     Volume:  -     ISSN:  1365-294X     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214478     Medline TA:  Mol Ecol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2011 Blackwell Publishing Ltd.
Affiliation:
Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ 08544, USA Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Difference in initial dental biofilm accumulation between night and day.
Next Document:  Molecular Dynamics Simulation of Tri-n-butyl-Phosphate Liquid: A Force Field Comparative Study.