| The evaluation of apoptosis and reactive oxygen species in hematopoietic cells derived from heparinized cadaveric organ donors stored short-term at 4 degrees C. | |
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MedLine Citation:
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PMID: 15964399 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Heparinized cadaveric organ donors are an important source of human organs and potentially of hematopoietic cells for transplantation purposes. The aim of this study was to evaluate the kinetics of programmed cell death in the hematopoietic cells harvested from these individuals and stored short-term at 4 degrees C. We also attempted to assess hematopoietic cell oxidation by measuring reactive oxygen species (ROS) generated by the mitochondria of stored cells. We found that these bone marrow cells harvested and stored at 4 degrees C for 7 days did not display a significant increase in programmed cell death. However, prolonged storage resulted in lower ROS production, indirectly giving evidence of activation of intracellular signaling proteins. |
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Authors:
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B Machaliński; M Baśkiewicz-Masiuk; K Pabisiak; K Grymula; J Sieńko; L Domański; M Ostrowski; A Machalińska |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Transplantation proceedings Volume: 37 ISSN: 0041-1345 ISO Abbreviation: Transplant. Proc. Publication Date: 2005 Jun |
Date Detail:
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Created Date: 2005-06-20 Completed Date: 2005-10-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0243532 Medline TA: Transplant Proc Country: United States |
Other Details:
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Languages: eng Pagination: 2281-4 Citation Subset: IM |
Affiliation:
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Department of General Pathology, Pomeranian Medical University, Al. Powstańców Wlkp. 72, 70-111 Szczecin, Poland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Apoptosis / physiology* Bone Marrow Cells / cytology* Brain Death Cadaver Female Flow Cytometry Hematopoietic Stem Cells* / cytology, physiology Humans Male Middle Aged Reactive Oxygen Species / metabolism* Tissue Donors Tissue Preservation / methods* Tissue and Organ Harvesting |
| Chemical | |
Reg. No./Substance:
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0/Reactive Oxygen Species |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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