| An essential role for transcription before the MBT in Xenopus laevis. | |
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MedLine Citation:
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PMID: 21741375 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Most zygotic genes remain transcriptionally silent in Drosophila, Xenopus, and zebrafish embryos through multiple mitotic divisions until the midblastula transition (MBT). Several genes have been identified in each of these organisms that are transcribed before the MBT, but whether precocious expression of specific mRNAs is important for later development has not been examined in detail. Here, we identify a class of protein coding transcripts activated before the MBT by the maternal T-box factor VegT that are components of an established transcriptional regulatory network required for mesendoderm induction in Xenopus laevis, including the Nodal related ligands xnr5, xnr6, and derrière and the transcription factors bix4, and sox17α. Accumulation of phospho-Smad2, a hallmark of active Nodal signaling, at the onset of the MBT requires preMBT transcription and activity of xnr5 and xnr6. Furthermore, preMBT activation of the Nodal pathway is essential for mesendodermal gene expression and patterning of the embryo. Finally, xnr5 and xnr6 can also activate their own expression during cleavage stages, indicating that preMBT transcription contributes to a feed-forward system that allows robust activation of Nodal signaling at the MBT. |
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Authors:
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Jennifer Skirkanich; Guillaume Luxardi; Jing Yang; Laurent Kodjabachian; Peter S Klein |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-06-29 |
Journal Detail:
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Title: Developmental biology Volume: 357 ISSN: 1095-564X ISO Abbreviation: Dev. Biol. Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-08-30 Completed Date: 2011-10-25 Revised Date: 2013-05-24 |
Medline Journal Info:
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Nlm Unique ID: 0372762 Medline TA: Dev Biol Country: United States |
Other Details:
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Languages: eng Pagination: 478-91 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
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Cell and Molecular Biology Graduate Group, University of Pennsylvania, Philadelphia, PA, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blastula / cytology, embryology*, metabolism* Embryo, Nonmammalian / metabolism Endoderm / embryology Feedback, Physiological Gene Expression Regulation, Developmental Nodal Protein / genetics, metabolism Signal Transduction / genetics Smad Proteins / metabolism Transcription, Genetic* Xenopus Proteins / genetics, metabolism Xenopus laevis / embryology*, genetics* |
| Grant Support | |
ID/Acronym/Agency:
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1R01GM076621/GM/NIGMS NIH HHS; R01 GM076621-01A1/GM/NIGMS NIH HHS; R01 GM076621-02/GM/NIGMS NIH HHS; R01 GM076621-03/GM/NIGMS NIH HHS; R01 GM076621-04/GM/NIGMS NIH HHS; R01 GM093217/GM/NIGMS NIH HHS; R01 GM093217-02/GM/NIGMS NIH HHS; R01GM093217/GM/NIGMS NIH HHS; T32-GM07229/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Nodal Protein; 0/Smad Proteins; 0/Xenopus Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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