Document Detail


An essential role for dermal primary cilia in hair follicle morphogenesis.
MedLine Citation:
PMID:  18987668     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The primary cilium is a microtubule-based organelle implicated as an essential component of a number of signaling pathways. It is present on cells throughout the mammalian body; however, its functions in most tissues remain largely unknown. Herein we demonstrate that primary cilia are present on cells in murine skin and hair follicles throughout morphogenesis and during hair follicle cycling in postnatal life. Using the Cre-lox system, we disrupted cilia assembly in the ventral dermis and evaluated the effects on hair follicle development. Mice with disrupted dermal cilia have severe hypotrichosis (lack of hair) in affected areas. Histological analyses reveal that most follicles in the mutants arrest at stage 2 of hair development and have small or absent dermal condensates. This phenotype is reminiscent of that seen in the skin of mice lacking Shh or Gli2. In situ hybridization and quantitative RT-PCR analysis indicates that the hedgehog pathway is downregulated in the dermis of the cilia mutant hair follicles. Thus, these data establish cilia as a critical signaling component required for normal hair morphogenesis and suggest that this organelle is needed on cells in the dermis for reception of signals such as sonic hedgehog.
Authors:
Jonathan M Lehman; Essam Laag; Edward J Michaud; Bradley K Yoder
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-11-06
Journal Detail:
Title:  The Journal of investigative dermatology     Volume:  129     ISSN:  1523-1747     ISO Abbreviation:  J. Invest. Dermatol.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-16     Completed Date:  2009-02-27     Revised Date:  2014-09-17    
Medline Journal Info:
Nlm Unique ID:  0426720     Medline TA:  J Invest Dermatol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  438-48     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cilia / physiology*
Dermis / cytology
Hair Follicle / cytology*,  growth & development*
Hedgehog Proteins / genetics,  metabolism*
Hypotrichosis / metabolism,  pathology,  physiopathology*
Integrases / genetics
Kruppel-Like Transcription Factors / genetics,  metabolism
Mice
Mice, Inbred C57BL
Mice, Knockout
Phenotype
Signal Transduction / physiology
Wnt Proteins / metabolism
Grant Support
ID/Acronym/Agency:
AR052792/AR/NIAMS NIH HHS; R01 AR052792/AR/NIAMS NIH HHS; R01 AR052792-01A2/AR/NIAMS NIH HHS; R01 HD056030/HD/NICHD NIH HHS; R01 HD056030-01A1/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Gli2 protein; 0/Hedgehog Proteins; 0/Kruppel-Like Transcription Factors; 0/Shh protein, mouse; 0/Wnt Proteins; EC 2.7.7.-/Cre recombinase; EC 2.7.7.-/Integrases
Comments/Corrections
Comment In:
J Invest Dermatol. 2009 Feb;129(2):264-5   [PMID:  19148215 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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