Document Detail


An essential role for IL-13 in maintaining a non-healing response following Leishmania mexicana infection.
MedLine Citation:
PMID:  12355446     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A comparison of the growth of Leishmania mexicana in IL-4(-/-), IL-4Ralpha(-/-) and wild-type BALB/c mice demonstrated a disease exacerbative role for IL-13 as well as IL-4. Thus, while both IL-4(-/-) and IL-4Ralpha(-/-) mice were more resistant than wild-type controls to infection with L. mexicana, IL-4Ralpha(-/-) mice, which are unresponsive to IL-13 as well as IL-4, were significantly more resistant to parasite growth than their IL-4(-/-) counterparts. Cytokine and antibody analysis revealed a Th1-biased specific response in both infected IL-4(-/-) and IL-4Ralpha(-/-) mice compared with wild-type animals. Reconstituting SCID mice with IL-4(-/-), IL-4Ralpha(-/-) or wild-type splenocytes prior to infection demonstrated that the early onset of lesion growth was dependent on the presence of lymphocytes responding to IL-4 and/or IL-13, as lesions failed to develop in only the SCID IL-4Ralpha(-/-) reconstituted mice. An independent role for IL-13 in L. mexicana infection was demonstrated by comparing disease progression in IL-13(-/-), IL-4(-/-)/IL-13(-/-) and wild-type B6/129 mice. In contrast to IL-4(-/-)/IL-13(-/-) mice, which were resistant, IL-13(-/-) mice developed lesions similar in size to wild-type animals up to week 8 post-infection. However, in contrast to wild-type mice in which disease continued to progress, lesions eventually healed in IL-13(-/-) mice, in association with the development of a Th1 response. Collectively our results suggest that IL-4 plays a critical role in early lesion development, and that IL-13 plays a crucial part in maintaining a chronic non-healing infection.
Authors:
James Alexander; Frank Brombacher; H Adrienne McGachy; Andrew N J McKenzie; William Walker; K Christine Carter
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of immunology     Volume:  32     ISSN:  0014-2980     ISO Abbreviation:  Eur. J. Immunol.     Publication Date:  2002 Oct 
Date Detail:
Created Date:  2002-09-30     Completed Date:  2002-11-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1273201     Medline TA:  Eur J Immunol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2923-33     Citation Subset:  IM    
Affiliation:
Department of Immunology, University of Strathclyde, Strathclyde Institute for Biomedical Sciences, 27 Taylor Street, Glasgow G4 0NR, Scotland, GB, UK. j.alexander@strath.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Protozoan / biosynthesis
Cytokines / biosynthesis,  genetics
Interleukin-13 / physiology*
Interleukin-4 / physiology
Leishmania mexicana / growth & development*,  immunology
Leishmaniasis, Cutaneous / immunology*
Lymphocyte Activation
Mice
Mice, Inbred BALB C
Mice, SCID
Receptors, Interleukin-4 / physiology
Chemical
Reg. No./Substance:
0/Antibodies, Protozoan; 0/Cytokines; 0/Interleukin-13; 0/Receptors, Interleukin-4; 207137-56-2/Interleukin-4

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