| An essential role for IL-13 in maintaining a non-healing response following Leishmania mexicana infection. | |
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MedLine Citation:
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PMID: 12355446 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A comparison of the growth of Leishmania mexicana in IL-4(-/-), IL-4Ralpha(-/-) and wild-type BALB/c mice demonstrated a disease exacerbative role for IL-13 as well as IL-4. Thus, while both IL-4(-/-) and IL-4Ralpha(-/-) mice were more resistant than wild-type controls to infection with L. mexicana, IL-4Ralpha(-/-) mice, which are unresponsive to IL-13 as well as IL-4, were significantly more resistant to parasite growth than their IL-4(-/-) counterparts. Cytokine and antibody analysis revealed a Th1-biased specific response in both infected IL-4(-/-) and IL-4Ralpha(-/-) mice compared with wild-type animals. Reconstituting SCID mice with IL-4(-/-), IL-4Ralpha(-/-) or wild-type splenocytes prior to infection demonstrated that the early onset of lesion growth was dependent on the presence of lymphocytes responding to IL-4 and/or IL-13, as lesions failed to develop in only the SCID IL-4Ralpha(-/-) reconstituted mice. An independent role for IL-13 in L. mexicana infection was demonstrated by comparing disease progression in IL-13(-/-), IL-4(-/-)/IL-13(-/-) and wild-type B6/129 mice. In contrast to IL-4(-/-)/IL-13(-/-) mice, which were resistant, IL-13(-/-) mice developed lesions similar in size to wild-type animals up to week 8 post-infection. However, in contrast to wild-type mice in which disease continued to progress, lesions eventually healed in IL-13(-/-) mice, in association with the development of a Th1 response. Collectively our results suggest that IL-4 plays a critical role in early lesion development, and that IL-13 plays a crucial part in maintaining a chronic non-healing infection. |
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Authors:
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James Alexander; Frank Brombacher; H Adrienne McGachy; Andrew N J McKenzie; William Walker; K Christine Carter |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of immunology Volume: 32 ISSN: 0014-2980 ISO Abbreviation: Eur. J. Immunol. Publication Date: 2002 Oct |
Date Detail:
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Created Date: 2002-09-30 Completed Date: 2002-11-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1273201 Medline TA: Eur J Immunol Country: Germany |
Other Details:
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Languages: eng Pagination: 2923-33 Citation Subset: IM |
Affiliation:
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Department of Immunology, University of Strathclyde, Strathclyde Institute for Biomedical Sciences, 27 Taylor Street, Glasgow G4 0NR, Scotland, GB, UK. j.alexander@strath.ac.uk |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Protozoan / biosynthesis Cytokines / biosynthesis, genetics Interleukin-13 / physiology* Interleukin-4 / physiology Leishmania mexicana / growth & development*, immunology Leishmaniasis, Cutaneous / immunology* Lymphocyte Activation Mice Mice, Inbred BALB C Mice, SCID Receptors, Interleukin-4 / physiology |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Protozoan; 0/Cytokines; 0/Interleukin-13; 0/Receptors, Interleukin-4; 207137-56-2/Interleukin-4 |
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