| Erp1/Emi2 is essential for the meiosis I to meiosis II transition in Xenopus oocytes. | |
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MedLine Citation:
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PMID: 17141208 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Erp1 (also called Emi2), an inhibitor of the APC/C ubiquitin ligase, is a key component of cytostatic factor (CSF) responsible for Meta-II arrest in vertebrate eggs. Reportedly, however, Erp1 is expressed even during meiosis I in Xenopus oocytes. If so, it is a puzzle why normally maturing oocytes cannot arrest at Meta-I. Here, we show that actually Erp1 synthesis begins only around the end of meiosis I in Xenopus oocytes, and that specific inhibition of Erp1 synthesis by morpholino oligos prevents entry into meiosis II. Furthermore, we demonstrate that premature, ectopic expression of Erp1 at physiological Meta-II levels can arrest maturing oocytes at Meta-I. Thus, our results show the essential role for Erp1 in the meiosis I/meiosis II transition in Xenopus oocytes and can explain why normally maturing oocytes cannot arrest at Meta-I. |
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Authors:
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Munemichi Ohe; Daigo Inoue; Yoshinori Kanemori; Noriyuki Sagata |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2006-11-03 |
Journal Detail:
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Title: Developmental biology Volume: 303 ISSN: 0012-1606 ISO Abbreviation: Dev. Biol. Publication Date: 2007 Mar |
Date Detail:
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Created Date: 2007-02-19 Completed Date: 2007-04-09 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0372762 Medline TA: Dev Biol Country: United States |
Other Details:
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Languages: eng Pagination: 157-64 Citation Subset: IM |
Affiliation:
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Department of Biology, Graduate School of Sciences, Kyushu University, Hakozaki 6-10-1, Fukuoka 812-8581, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle Proteins / metabolism F-Box Proteins / metabolism* Gene Expression Regulation, Developmental* Immunoblotting Immunohistochemistry Meiosis / physiology* Oligonucleotides Oocytes / physiology* Protein Kinases / metabolism Xenopus / physiology* Xenopus Proteins / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/Erp1 protein, Xenopus; 0/F-Box Proteins; 0/Oligonucleotides; 0/Xenopus Proteins; EC 2.7.-/Protein Kinases; EC 2.7.1.-/cdc2 protein, Xenopus |
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