Document Detail

Erp1/Emi2 is essential for the meiosis I to meiosis II transition in Xenopus oocytes.
MedLine Citation:
PMID:  17141208     Owner:  NLM     Status:  MEDLINE    
Erp1 (also called Emi2), an inhibitor of the APC/C ubiquitin ligase, is a key component of cytostatic factor (CSF) responsible for Meta-II arrest in vertebrate eggs. Reportedly, however, Erp1 is expressed even during meiosis I in Xenopus oocytes. If so, it is a puzzle why normally maturing oocytes cannot arrest at Meta-I. Here, we show that actually Erp1 synthesis begins only around the end of meiosis I in Xenopus oocytes, and that specific inhibition of Erp1 synthesis by morpholino oligos prevents entry into meiosis II. Furthermore, we demonstrate that premature, ectopic expression of Erp1 at physiological Meta-II levels can arrest maturing oocytes at Meta-I. Thus, our results show the essential role for Erp1 in the meiosis I/meiosis II transition in Xenopus oocytes and can explain why normally maturing oocytes cannot arrest at Meta-I.
Munemichi Ohe; Daigo Inoue; Yoshinori Kanemori; Noriyuki Sagata
Related Documents :
19881348 - Maternal age and chromosomally abnormal pregnancies: what we know and what we wish we k...
8819998 - Oocyte donation to women over 40 years of age: pregnancy complications.
17350188 - Effect of individual heifer oocyte donors on cloned embryo development in vitro.
10783368 - Chemically and mechanically induced membrane fusion: non-activating methods for nuclear...
19858748 - Reproductive history and adverse pregnancy outcomes in commercial flight crew and air t...
18199728 - Cord serum estrogens, androgens, insulin-like growth factor-i, and insulin-like growth ...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-11-03
Journal Detail:
Title:  Developmental biology     Volume:  303     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-02-19     Completed Date:  2007-04-09     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  157-64     Citation Subset:  IM    
Department of Biology, Graduate School of Sciences, Kyushu University, Hakozaki 6-10-1, Fukuoka 812-8581, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Cycle Proteins / metabolism
F-Box Proteins / metabolism*
Gene Expression Regulation, Developmental*
Meiosis / physiology*
Oocytes / physiology*
Protein Kinases / metabolism
Xenopus / physiology*
Xenopus Proteins / metabolism*
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Erp1 protein, Xenopus; 0/F-Box Proteins; 0/Oligonucleotides; 0/Xenopus Proteins; EC 2.7.-/Protein Kinases; EC 2.7.1.-/cdc2 protein, Xenopus

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Negative association of obesity and its related chronic inflammation with serum glycated albumin but...
Next Document:  The anaphase-promoting complex is required in both dividing and quiescent cells during zebrafish dev...