Document Detail


Erp1/Emi2 is essential for the meiosis I to meiosis II transition in Xenopus oocytes.
MedLine Citation:
PMID:  17141208     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Erp1 (also called Emi2), an inhibitor of the APC/C ubiquitin ligase, is a key component of cytostatic factor (CSF) responsible for Meta-II arrest in vertebrate eggs. Reportedly, however, Erp1 is expressed even during meiosis I in Xenopus oocytes. If so, it is a puzzle why normally maturing oocytes cannot arrest at Meta-I. Here, we show that actually Erp1 synthesis begins only around the end of meiosis I in Xenopus oocytes, and that specific inhibition of Erp1 synthesis by morpholino oligos prevents entry into meiosis II. Furthermore, we demonstrate that premature, ectopic expression of Erp1 at physiological Meta-II levels can arrest maturing oocytes at Meta-I. Thus, our results show the essential role for Erp1 in the meiosis I/meiosis II transition in Xenopus oocytes and can explain why normally maturing oocytes cannot arrest at Meta-I.
Authors:
Munemichi Ohe; Daigo Inoue; Yoshinori Kanemori; Noriyuki Sagata
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-11-03
Journal Detail:
Title:  Developmental biology     Volume:  303     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-02-19     Completed Date:  2007-04-09     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  157-64     Citation Subset:  IM    
Affiliation:
Department of Biology, Graduate School of Sciences, Kyushu University, Hakozaki 6-10-1, Fukuoka 812-8581, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle Proteins / metabolism
F-Box Proteins / metabolism*
Gene Expression Regulation, Developmental*
Immunoblotting
Immunohistochemistry
Meiosis / physiology*
Oligonucleotides
Oocytes / physiology*
Protein Kinases / metabolism
Xenopus / physiology*
Xenopus Proteins / metabolism*
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Erp1 protein, Xenopus; 0/F-Box Proteins; 0/Oligonucleotides; 0/Xenopus Proteins; EC 2.7.-/Protein Kinases; EC 2.7.1.-/cdc2 protein, Xenopus

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