Document Detail


Emw1p/YNL313cp is essential for maintenance of the cell wall in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  21273246     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
There are six essential genes in the S. cerevisae genome which encode proteins bearing the tetratricopeptide repeat (TPR) domain that mediates protein-protein interaction. Thus far, the function of one of them, YNL313c, remains unknown. Our conditional mutants of YNL313c display osmoremedial temperature sensitivity, hypersensitivity to both Calcofluor White and low concentrations of SDS, and osmoremedial caffeine sensitivity. These are hallmarks of mutants that display cell wall defects. Accordingly we re-name the gene as EMW1 (essential for maintenance of the cell wall). Loss of Emw1p function is not associated with abrogation of the cell wall integrity (CWI) MAP kinase cascade. Instead, emw1ts mutants activate this cascade even at permissive temperature, indicating that loss of Emw1p function does not cause a defect in sensors and effectors of cell wall signaling, but leads to a cell wall defect directly. Constitutive activation of the CWI cascade is reflected by the overproduction of chitin by emw1ts mutants, a compensatory response frequently displayed by cell wall mutants. Growth is restored to emw1ts mutants incubated at otherwise non-permissive temperature when GFA1 is overexpressed. GFA1 encodes the hexosephosphate aminotransferase that catalyses the rate limiting step in the pathway that synthesizes the chitin precursor UDP-N-acetylglucosamine (UDP-GlcNAc). The possibility that Emw1p is required for function of Gfa1p was ruled out because the emw1ts phenotype persists when the requirement for Gfa1p is bypassed. Furthermore, if loss of Emw1p function leads to loss of function of Gfa1p, then chitin synthesis would be diminished. Instead, a stimulation in synthesis of this polymer is detected. Consequently, the defect associated with emw1ts mutants may be associated with compromise in one of the remaining processes that depend on UDP-GlcNAc, namely N-glycosylation or glycosylphosphatidylinositol (GPI) anchor synthesis.
Authors:
Tanya Sipling; Chao Zhai; Barry Panaretou
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-1-27
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  -     ISSN:  1465-2080     ISO Abbreviation:  -     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-1-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
King's College London;
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