Document Detail

An essential gene in Saccharomyces cerevisiae shares an upstream regulatory element with PRP4.
MedLine Citation:
PMID:  2204247     Owner:  NLM     Status:  MEDLINE    
ORF2 is an essential gene immediately upstream of PRP4 (formerly RNA4), a gene involved in nuclear mRNA processing in Saccharomyces cerevisiae. The two genes are arranged head-to-head. An 8 base-pair conserved sequence element is found upstream of both genes, as well as upstream of certain other genes that are known to be involved in pre-mRNA processing. Through deletion analysis we have found that both of the conserved sequence elements are important for transcription of both genes. We have cloned ORF2 and have isolated temperature-sensitive orf2 mutants. The phenotype of these mutants does not suggest a role for ORF2 in mRNA processing. The deduced amino acid sequence of ORF2 indicates significant similarity to DPR1, a gene encoding a protein that is involved in the carboxy-terminal processing of G-protein.
S Petersen-Bjørn; T R Harrington; J D Friesen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Yeast (Chichester, England)     Volume:  6     ISSN:  0749-503X     ISO Abbreviation:  Yeast     Publication Date:    1990 Jul-Aug
Date Detail:
Created Date:  1990-10-11     Completed Date:  1990-10-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8607637     Medline TA:  Yeast     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  345-52     Citation Subset:  IM    
Hospital for Sick Children, Toronto, Ontario, Canada.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Cell Membrane / metabolism
Cloning, Molecular
Fungal Proteins / genetics
Genes, Fungal*
Molecular Sequence Data
RNA Processing, Post-Transcriptional / genetics*
RNA, Fungal / genetics,  metabolism
RNA, Messenger / metabolism
Regulatory Sequences, Nucleic Acid*
Saccharomyces cerevisiae / genetics*,  metabolism
Transcription, Genetic
Reg. No./Substance:
0/Fungal Proteins; 0/RNA, Fungal; 0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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