| An essential gene for fruiting body initiation in the basidiomycete Coprinopsis cinerea is homologous to bacterial cyclopropane fatty acid synthase genes. | |
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MedLine Citation:
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PMID: 16322509 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The self-compatible Coprinopsis cinerea homokaryon AmutBmut produces fruiting bodies without prior mating to another strain. Early stages of fruiting body development include the dark-dependent formation of primary hyphal knots and their light-induced transition to the more compact secondary hyphal knots. The AmutBmut UV mutant 6-031 forms primary hyphal knots, but development arrests at the transition state by a recessive defect in the cfs1 gene, isolated from a cosmid library by mutant complementation. A normal primordia phenotype was achieved when cfs1+ was embedded at both sides in at least 4.0 kb of native flanking DNA. Truncations of the flanking DNA lead to reduction in transformation frequencies and faults in primordia tissue formation, suggesting that the gene is also acting at later stages of development. The cfs1 gene encodes a protein highly similar to cyclopropane fatty acid synthases, a class of enzymes shown in prokaryotes and recently in a plant to convert membrane-bound unsaturated fatty acids into cyclopropane fatty acids. In C. cinerea 6-031, the mutant cfs1 allele carries a T-to-G transversion, leading to an amino acid substitution (Y441D) in a domain suggested to be involved in the catalytic function of the protein and/or membrane interaction. |
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Authors:
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Yi Liu; Prayook Srivilai; Sabine Loos; Markus Aebi; Ursula Kües |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2005-12-01 |
Journal Detail:
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Title: Genetics Volume: 172 ISSN: 0016-6731 ISO Abbreviation: Genetics Publication Date: 2006 Feb |
Date Detail:
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Created Date: 2006-02-28 Completed Date: 2006-06-20 Revised Date: 2013-06-07 |
Medline Journal Info:
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Nlm Unique ID: 0374636 Medline TA: Genetics Country: United States |
Other Details:
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Languages: eng Pagination: 873-84 Citation Subset: IM |
Affiliation:
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Institute for Microbiology, ETH Zurich, Switzerland. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AF338438 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Agaricales
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genetics*,
growth & development Amino Acid Sequence Cosmids Fruiting Bodies, Fungal / genetics*, growth & development Genes, Bacterial* Genes, Fungal* Methyltransferases / genetics* Molecular Sequence Data Mutation Sequence Homology, Amino Acid* Ultraviolet Rays |
| Chemical | |
Reg. No./Substance:
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EC 2.1.1.-/Methyltransferases; EC 2.1.1.-/cyclopropane synthetase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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