Document Detail


The essential function of Swc4p - a protein shared by two chromatin-modifying complexes of the yeast Saccharomyces cerevisiae - resides within its N-terminal part.
MedLine Citation:
PMID:  18726008     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Swc4p protein, encoded by an essential gene, is shared by two chromatin-remodeling complexes in Saccharomyces cerevisiae cells: NuA4 (nucleosome acetyltransferase of H4) and SWR1. The SWR1 complex catalyzes ATP-dependent exchange of the nucleosomal histone H2A for H2AZ (Htz1p). The activity of NuA4 is responsible mainly for the acetylation of the H4 histone but also for the acetylation of H2A and H2AZ. In this work we investigated the role of the Swc4p protein. Using random mutagenesis we isolated a collection of swc4 mutants and showed that the essential function of Swc4p resides in its N-terminal part, within the first 269 amino acids of the 476-amino acid-long protein. We also demonstrated that Swc4p is able to accommodate numerous mutations without losing its functionality under standard growth conditions. However, when swc4 mutants were exposed to methyl methanesulfonate (MMS), hydroxyurea or benomyl, severe growth deficiencies appeared, pointing to an involvement of Swc4p in many chromatin-based processes. The mutants' phenotypes did not result from an impairment of histone acetylation, as in the mutant which bears the shortest isolated variant of truncated Swc4p, the level of overall H4 acetylation was unchanged.
Authors:
Arkadiusz Miciałkiewicz; Anna Chełstowska
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-25
Journal Detail:
Title:  Acta biochimica Polonica     Volume:  55     ISSN:  0001-527X     ISO Abbreviation:  Acta Biochim. Pol.     Publication Date:  2008  
Date Detail:
Created Date:  2008-10-15     Completed Date:  2009-02-04     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  14520300R     Medline TA:  Acta Biochim Pol     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  603-12     Citation Subset:  IM    
Affiliation:
Department of Genetics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland.
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MeSH Terms
Descriptor/Qualifier:
Acetylation
Alleles
Amino Acid Sequence
Base Sequence
Chromatin Assembly and Disassembly
DNA Primers / genetics
DNA, Fungal / genetics
Genes, Fungal
Histone Acetyltransferases
Histones / chemistry
Molecular Sequence Data
Mutagenesis
Peptide Fragments / chemistry,  genetics,  metabolism
Phenotype
Saccharomyces cerevisiae / genetics,  metabolism*
Saccharomyces cerevisiae Proteins / chemistry*,  genetics,  metabolism*
Sequence Deletion
Sequence Homology, Amino Acid
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Fungal; 0/Histones; 0/Peptide Fragments; 0/Saccharomyces cerevisiae Proteins; 0/Swc4 protein, S cerevisiae; EC 2.3.1.48/Histone Acetyltransferases

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